Mansilla E, Marín G H, Drago H, Sturla F, Salas E, Gardiner C, Bossi S, Lamonega R, Guzmán A, Nuñez A, Gil M A, Piccinelli G, Ibar R, Soratti C
Centro Unico Coordinador de Ablación e Implante de Buenos Aires (CUCAIBA), Ministry of Health, Buenos Aires, Argentina.
Transplant Proc. 2006 Apr;38(3):967-9. doi: 10.1016/j.transproceed.2006.02.053.
Recent work has shown that human bone marrow contains mesenchymal stem cells (MSCs). However, little is known about their presence in peripheral blood. Since these cells are potentially responsible for tissue repair after injury, their number should be increased during these situations. To demonstrate their number during these situations, we measured MSCs in the peripheral blood of healthy donors and burn patients.
Blood samples were obtained from 15 acute burn patients and 15 healthy donors. We performed flow cytometric analysis, using a large monoclonal antibody panel: CD44, CD45, CD14, DR, CD34, CD19, CD13, CD29, CD105, CD1a, CD90, CD38, CD25. MSC phenotype was considered positive for CD44, CD13, CD29, CD90, and CD105, and negative for the other monoclonals. The testing was performed on day 3 after injury. We correlated the results with the age, sex, and size and type of burns.
Cells expressing the MSC phenotype were detected in the peripheral blood of both groups. Noteworthy, compared with samples from healthy donors (0.0078 +/- 0.0044), blood obtained from burn patients showed a higher MSC percentage (0.1643 +/- 0.115; P < .001). The percentage of MSCs correlated with the size and severity of the burn. Increased values were also observed among younger patients.
MSCs have an important role in regenerative processes of human tissues. We found cells phenotypically identical to MSCs circulating in physiological number in normal subjects, but in significantly higher amounts during acute large burns. Therefore, they may represent a previously unrecognized circulatory component to the process of skin regeneration.
近期研究表明人类骨髓中含有间充质干细胞（MSC）。然而，对于其在外周血中的存在情况却知之甚少。由于这些细胞可能在损伤后负责组织修复，因此在这些情况下它们的数量应该会增加。为了证明在这些情况下它们的数量，我们检测了健康供体和烧伤患者外周血中的MSC。
采集了15例急性烧伤患者和15名健康供体的血样。我们使用一大组单克隆抗体进行流式细胞术分析：CD44、CD45、CD14、DR、CD34、CD19、CD13、CD29、CD105、CD1a、CD90、CD38、CD25。MSC表型被认为CD44、CD13、CD29、CD90和CD105呈阳性，而其他单克隆抗体呈阴性。检测在受伤后第3天进行。我们将结果与年龄、性别以及烧伤的面积和类型进行了关联分析。
两组外周血中均检测到表达MSC表型的细胞。值得注意的是，与健康供体的样本（0.0078±0.0044）相比，烧伤患者的血液中MSC百分比更高（0.1643±0.115；P<0.001）。MSC的百分比与烧伤的面积和严重程度相关。在年轻患者中也观察到数值升高。
MSC在人体组织的再生过程中发挥着重要作用。我们发现正常受试者中存在表型与MSC相同的细胞，其数量处于生理水平，但在急性大面积烧伤时数量显著增加。因此，它们可能是皮肤再生过程中一个此前未被认识的循环成分。
