Ertürk Ahmet, Demir Sabri, Günal Yasemin Dere, Zengin Mehmet, Çinar Miyase, Yildiz Dinçer, Karahan Siyami, Şenel Emrah
Department of Pediatric Surgery, Ankara City Hospital, Ankara, Turkey.
Department of Pediatric Surgery, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey.
Turk J Med Sci. 2021 Nov 9;52(2):505-513. doi: 10.3906/sag-2105-168. eCollection 2022.
BACKGROUND/AIM: The aim of this study was to investigate the healing effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental testicular torsion in rats.
Three groups consisting of 10 Wistar albino rats were created. In Group I, the left testicle was explored and relocated in the scrotum without any attempt to modify it. In Group II, the left testicle underwent torsion for three h and then was detorsed and relocated. In Group III, in addition to torsion and detorsion, BM-MSCs were administered intratesticularly. The rats were sacrificed on the seventh day, and the healing status of the testicles was investigated with histopathological and biochemical analyses. BM-MSC involvement was investigated by immunofluorescence microscopy. Statistical analysis was performed using SPSS 15.0. A p-value < 0.05 was considered statistically significant for all variables.
Immunofluorescence microscopy showed that BM-MSCs were located around the Leydig cells in Group III. Under light microscopy, the mean Johnsen Score of Group III was significantly higher than that of Group II (p = 0.035). The interleukin-10 (IL-10) level was significantly higher in Group III compared to Group II (p = 0.003). While the malondialdehyde (MDA) values in Group I (the control group) were lower than in the other groups (p = 0.037), the superoxide dismutase (SOD) values were similar (p = 0.158). Although there was no statistically significant difference between Group II and Group III in terms of MDA, it was lower in Group III. Although the tissue SOD levels were higher in Group III than in Group II, the difference was not statistically significant.
This study has demonstrated that BM-MSCs significantly corrected the Johnsen Score and increased anti-inflammatory cytokine levels after testicular torsion. BM-MSCs can be used in testicular torsion as supportive therapy to minimize tissue damage.
背景/目的：本研究旨在探讨骨髓间充质干细胞（BM-MSCs）对大鼠实验性睾丸扭转的愈合作用。
将30只Wistar白化大鼠分为三组，每组10只。第一组，探查左侧睾丸并将其重新置于阴囊内，未作任何处理。第二组，左侧睾丸扭转3小时，然后解除扭转并重新定位。第三组，除扭转和解除扭转外，还经睾丸内注射BM-MSCs。在第7天处死大鼠，通过组织病理学和生化分析研究睾丸的愈合情况。通过免疫荧光显微镜检查研究BM-MSCs的参与情况。使用SPSS 15.0进行统计分析。所有变量的p值<0.05被认为具有统计学意义。
免疫荧光显微镜检查显示，第三组的BM-MSCs位于睾丸间质细胞周围。在光学显微镜下，第三组的平均约翰森评分显著高于第二组（p = 0.035）。与第二组相比，第三组的白细胞介素10（IL-10）水平显著更高（p = 0.003）。第一组（对照组）的丙二醛（MDA）值低于其他组（p = 0.037），而超氧化物歧化酶（SOD）值相似（p = 0.158）。虽然第二组和第三组在MDA方面无统计学显著差异，但第三组的MDA较低。虽然第三组的组织SOD水平高于第二组，但差异无统计学意义。
本研究表明，BM-MSCs可显著改善睾丸扭转后的约翰森评分并提高抗炎细胞因子水平。BM-MSCs可作为支持性治疗用于睾丸扭转，以尽量减少组织损伤。
