慢性鼻窦炎小鼠模型的建立。
Development of a murine model of chronic rhinosinusitis.
作者信息
Lindsay Robin, Slaughter Tiffani, Britton-Webb Joy, Mog Steven R, Conran Rich, Tadros Monica, Earl Natalie, Fox David, Roberts John, Bolger William E
机构信息
National Naval Medical Center, and the Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889-5612, USA.
出版信息
Otolaryngol Head Neck Surg. 2006 May;134(5):724-30; discussion 731-2. doi: 10.1016/j.otohns.2005.11.048.
OBJECTIVE
The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis.
STUDY DESIGN
Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response.
SETTING
Animal care facility at an academic institution.
RESULTS
A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories.
CONCLUSION
Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis.
SIGNIFICANCE
A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology.
EBM RATING
B-2.
目的
本研究的目的是建立慢性嗜酸性粒细胞性鼻-鼻窦炎的小鼠模型。
研究设计
通过腹腔注射使小鼠对烟曲霉菌（Af）提取物致敏。随后，动物每周接受3次Af提取物滴鼻激发，持续12周。耳鼻喉科医生和病理学家对鼻窦复合体进行组织学研究，以描述炎症反应特征。
研究地点
一所学术机构的动物护理设施。
结果
所有研究动物均诱发了慢性嗜酸性粒细胞炎症反应。对炎症、分泌细胞增生、肥大细胞和嗜酸性粒细胞进行了统计分析。在所有类别中，对照小鼠和研究小鼠之间存在非常显著的差异（P<0.0005）。
结论
长时间用Af提取物滴鼻激发可在小鼠鼻粘膜中产生炎症反应，模拟人类慢性嗜酸性粒细胞性鼻-鼻窦炎。
意义
报道了一种慢性鼻-鼻窦炎的小鼠模型，可能有助于未来对疾病病理生理学的研究。
循证医学评级
B-2。
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