Ahn Byung-Hoon, Park Youn-Ho, Shin Seung-Heon
Department of Otolaryngology, School of Medicine, Keimyung University, South Korea.
Auris Nasus Larynx. 2009 Aug;36(4):422-6. doi: 10.1016/j.anl.2008.08.009. Epub 2008 Dec 11.
Fungi are known to induce the production of chemical mediators from respiratory epithelial cells and have been increasingly recognized as important pathogens in sinusitis. However, the exact role of fungi in the pathogenesis of rhinosinusitis has not been clearly established. This study was performed to improve our understanding of the role of fungi in the pathogenesis of rhinosinusitis by developing an animal model of fungus induced rhinosinusitis.
Fifty mice (C57BL6/J) were divided into five groups. Sham-operated group was the first group. In the second group, Aspergillus versicolor (Group IIa) and Alternaria alternata (Group IIb) (10(6)spores/ml) were inoculated into the nasal cavity. In the third group, fungi were inoculated into the nasal cavity in the presence of mucosal scratch (Group IIIa,b) and the fourth group was a nasal mucosal scratch only (Group IV). The fifth was a negative control (Group V). The fungi were inoculated once a week on six occasions and then the animals were sacrificed at 7 weeks. The histological sections were examined in a blind manner for the appearance of neutrophil clusters and epithelial thickness with hematoxylin-eosin stain, and mucus secreting glands using the Alcian blue/periodic acid Schiff stain.
Non-invasive fungal sinusitis had been induced with increased numbers of neutrophil clusters after Aspergillus and Alternaria exposure. The mice with the mucosal scratch wounds had significantly more inflammatory cell infiltration and epithelial thickening; but eosinophils were not commonly found. The mice with fungal sinusitis had goblet cell hyperplasia and increased mucus secretion in the sinonasal cavity.
Inoculation of fungi in the nasal cavity induced rhinosinusitis in C57BL6/J mice. This mouse model may be used for better understanding of the role of fungi in the pathogenesis of rhinosinusitis.
已知真菌可诱导呼吸道上皮细胞产生化学介质，并且越来越被认为是鼻窦炎的重要病原体。然而，真菌在鼻-鼻窦炎发病机制中的确切作用尚未明确。本研究旨在通过建立真菌诱导的鼻-鼻窦炎动物模型，增进我们对真菌在鼻-鼻窦炎发病机制中作用的理解。
将50只小鼠（C57BL6/J）分为五组。第一组为假手术组。第二组，将杂色曲霉（IIa组）和链格孢（IIb组）（10⁶孢子/毫升）接种到鼻腔。第三组，在存在黏膜划痕的情况下将真菌接种到鼻腔（IIIa、b组），第四组仅进行鼻黏膜划痕（IV组）。第五组为阴性对照组（V组）。每周接种一次真菌，共接种六次，然后在7周时处死动物。用苏木精-伊红染色对组织切片进行盲法检查，观察中性粒细胞簇的出现和上皮厚度，并用阿尔辛蓝/过碘酸希夫染色观察黏液分泌腺。
暴露于曲霉和链格孢后，诱导出了非侵袭性真菌性鼻窦炎，中性粒细胞簇数量增加。有黏膜划痕伤口的小鼠炎症细胞浸润和上皮增厚明显更严重；但未常见到嗜酸性粒细胞。患有真菌性鼻窦炎的小鼠杯状细胞增生，鼻窦腔内黏液分泌增加。
在鼻腔接种真菌可诱导C57BL6/J小鼠发生鼻-鼻窦炎。该小鼠模型可用于更好地理解真菌在鼻-鼻窦炎发病机制中的作用。
