Heppner T J, Fiekers J F
Department of Anatomy and Neurobiology, University of Vermont, College of Medicine, Burlington 05405.
Brain Res. 1991 Nov 1;563(1-2):303-5. doi: 10.1016/0006-8993(91)91551-b.
Soman, (pinacoloxymethyl-phosphoryl fluoride) (0.1-10 microM) an irreversible cholinesterase inhibitor, reversibly reduced the duration of calcium (Ca2+)- and barium (Ba2+) spikes without significantly affecting spike amplitude in sympathetic postganglionic neurons of the adult bullfrog (Rana catesbeiana). The soman-induced shortening of the spike duration was not prevented by pretreatment with either (+)-tubocurarine (100 microM) or hexamethonium (100 microM) and atropine (10 microM) and was also recorded from acutely-dissociated sympathetic neurons. These results suggest that soman has a direct action to decrease calcium entry through voltage-dependent channels activated during a spike. This effect may contribute to both the decrease in the duration of the spike after-hyperpolarization (AHP) and the enhanced neuronal excitability produced by soman in these neurons.
梭曼（频哪基甲氧基磷酰氟）（0.1 - 10微摩尔）是一种不可逆的胆碱酯酶抑制剂，可使成年牛蛙（牛蛙）交感神经节后神经元中钙（Ca2 +）和钡（Ba2 +）尖峰的持续时间可逆性缩短，而对尖峰幅度无显著影响。用（+） - 筒箭毒碱（100微摩尔）或六甲铵（100微摩尔）以及阿托品（10微摩尔）预处理并不能阻止梭曼诱导的尖峰持续时间缩短，并且在急性解离的交感神经元中也记录到了这种现象。这些结果表明，梭曼具有直接作用，可减少通过尖峰期间激活的电压依赖性通道的钙内流。这种效应可能有助于梭曼在这些神经元中引起的尖峰后超极化（AHP）持续时间的减少以及神经元兴奋性的增强。
