Ngawhirunpat T, Panomsuk S, Opanasopit P, Rojanarata T, Hatanaka T
Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Sanamchan Palace campus, Muang, Nakorn Pathom 73000, Thailand.
Pharmazie. 2006 Apr;61(4):331-5.
The in vitro transdermal permeation of eight hydrophilic drugs (antipyrine, L-dopa, dopamine hydrochloride, diclofenac sodium, 5-fluorouracil, isoprenaline hydrochloride, nicorandil and morphine hydrochloride) and eight lipophilic drugs (aminopyrine, cyclobarbital, ibuprofen, indomethacin, isosorbide dinitrate, flurbiprofen, ketoprofen and lignocaine) was determined using shed snake skin of Elaphae obsoleta and human skin. The permeation parameters and physiological characteristics of the skin, e.g. the water and lipid content, and the thickness of shed snake skin and human skin were evaluated and compared. In shed snake skin, the permeability coefficients (P) of lipophilic drugs were in the same range as those through the human skin (0.9 to 1.8-times); whereas those of hydrophilic drugs were remarkably lower (3.3 to 6.1-times). The thickness and lipid content of shed snake skin and human stratum corneum were not significantly different (P > 0.05), whereas the water content of shed snake skin was significantly lower than that of human stratum corneum (P < 0.05). The lower permeability of shed snake skin for hydrophilic compounds might be caused by the lower porosity of skin strata. The results suggested a potential use of shed snake skin as barrier membrane for lipophilic compounds percutaneous absorption studies in vitro.
使用王锦蛇的蜕下蛇皮和人皮肤，测定了8种亲水性药物（安替比林、左旋多巴、盐酸多巴胺、双氯芬酸钠、5-氟尿嘧啶、盐酸异丙肾上腺素、尼可地尔和盐酸吗啡）和8种亲脂性药物（氨基比林、环巴比妥、布洛芬、吲哚美辛、硝酸异山梨酯、氟比洛芬、酮洛芬和利多卡因）的体外透皮渗透情况。评估并比较了皮肤的渗透参数和生理特性，如水分和脂质含量，以及王锦蛇蜕下蛇皮和人皮肤的厚度。在王锦蛇蜕下蛇皮中，亲脂性药物的渗透系数（P）与通过人皮肤的渗透系数处于同一范围（0.9至1.8倍）；而亲水性药物的渗透系数则显著更低（3.3至6.1倍）。王锦蛇蜕下蛇皮和人角质层的厚度及脂质含量无显著差异（P>0.05），而王锦蛇蜕下蛇皮的水分含量显著低于人角质层（P<0.05）。王锦蛇蜕下蛇皮对亲水性化合物的较低渗透性可能是由于皮肤各层孔隙率较低所致。结果表明，王锦蛇蜕下蛇皮有潜力用作体外亲脂性化合物经皮吸收研究的屏障膜。
