在三元体系（嵌入剂-DNA-叶绿酸）中测得的叶绿酸的“拦截”特性。
The "interceptor" properties of chlorophyllin measured within the three-component system: intercalator-DNA-chlorophyllin.
作者信息
Pietrzak Monika, Wieczorek Zbigniew, Wieczorek Jolanta, Darzynkiewicz Zbigniew
机构信息
Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland.
出版信息
Biophys Chem. 2006 Aug 20;123(1):11-9. doi: 10.1016/j.bpc.2006.03.018. Epub 2006 Apr 6.
Abstract
In aqueous solutions, in the presence of double-stranded DNA, chlorophyllin (CHL) forms complexes with each of the three DNA intercalators: acridine orange (AO), quinacrine mustard (QM), and doxorubicin (DOX). The evidence for these interactions was obtained by measurement changes in the absorption and fluorescence spectra of the mixtures containing DNA and intercalators during titration with CHL. A model of simple competition between DNA and CHL for the intercalator was used to define the measured interactions. The concentrations of the complexes estimated based on this model were consistent with the concentrations obtained by actual measurement of the absorption spectra. The present data provide further support for the role of chlorophyllin as an "interceptor" that may neutralize biological activity of aromatic compounds including mutagens and antitumor drugs.
摘要
在水溶液中，在双链DNA存在的情况下，叶绿酸（CHL）与三种DNA嵌入剂中的每一种形成复合物：吖啶橙（AO）、喹吖因氮芥（QM）和阿霉素（DOX）。通过测量在用CHL滴定期间含有DNA和嵌入剂的混合物的吸收光谱和荧光光谱的变化，获得了这些相互作用的证据。使用DNA和CHL对嵌入剂的简单竞争模型来定义所测量的相互作用。基于该模型估计的复合物浓度与通过实际测量吸收光谱获得的浓度一致。目前的数据进一步支持了叶绿酸作为“拦截器”的作用，它可能中和包括诱变剂和抗肿瘤药物在内的芳香族化合物的生物活性。
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