Furuya Minoru, Tsushima Yu, Tani Shinobu, Kamimura Takashi
Pharmaceutical Discovery Research Laboratories, Teijin Institute for Bio-medical Research, Teijin Pharma Ltd, 4-3-2 Asahigaoka, Hino, Tokyo 191-8512, Japan.
Bioorg Med Chem. 2006 Aug 1;14(15):5093-8. doi: 10.1016/j.bmc.2006.04.020. Epub 2006 May 2.
By repeatedly introducing hydrophilic polyethylene glycol (PEG) spacer (2) onto affinity resin bearing a bioactive peptide (1/2 secretory leukocyte protease inhibitor, 1/2SLPI) as a ligand, the adsorption of nonspecific binding proteins was effectively reduced and the purification efficacy of elastase, which is one of the target molecules for 1/2SLPI, from a protein mixture was improved. Moreover, using this resin, we also successfully detected L-plastin, as an endogenous target molecule for SLPI, from HL-60 cell lysate.
