Baker R E, Ruijter J M, Walden J, Bingmann D, Straub H, Speckmann E J
Netherlands Institute for Brain Research, Amsterdam.
Neurosci Lett. 1991 Sep 30;131(1):61-5. doi: 10.1016/0304-3940(91)90337-s.
Long-term cultures of organotypic neonatal rat neocortex slices were maintained in a serum-free medium supplemented with 25 mM potassium. Such cultures continue to be bioelectrically silent upon return to a 5 mM potassium medium. The absence of responses to pressure ejected gamma-aminobutyric acid (GABA) and N-methyl-D-aspartate (NMDA) from neurons in treated explants suggests that one consequence of chronic depolarization is a reduction in the density of postsynaptic transmitter receptors. [3H]Muscimol binding to neocortical membrane preparations shows a large reduction in the binding of this agonist to GABAA receptors. These data show that the quantitative expression of at least one neurotransmitter receptor, the GABAA receptor, relies on voltage-dependent activity in developing neocortical neurons in vitro.
将新生大鼠新皮质切片进行器官型长期培养，培养条件为添加25 mM钾离子的无血清培养基。当恢复到5 mM钾离子培养基时，此类培养物在生物电方面仍保持沉默。经处理的外植体中的神经元对压力喷射的γ-氨基丁酸（GABA）和N-甲基-D-天冬氨酸（NMDA）无反应，这表明慢性去极化的一个后果是突触后递质受体密度降低。[3H]蝇蕈醇与新皮质膜制剂的结合显示，该激动剂与GABAA受体的结合大幅减少。这些数据表明，至少一种神经递质受体即GABAA受体的定量表达依赖于体外发育中的新皮质神经元的电压依赖性活动。
