在使用放射性前体对植物组织进行短期标记期间，磷酸脂酸和磷脂酰肌醇 4,5-二磷酸的表观水平会人为升高。
Artifactual Elevation of the Apparent Levels of Phosphatidic Acid and Phosphatidylinositol 4,5-Bisphosphate during Short-Term Labeling of Plant Tissue with Radioactive Precursor.
机构信息
Department of Molecular and Cell Biology, U-125, University of Connecticut, Storrs, Connecticut 06269-3125.
出版信息
Plant Physiol. 1992 Oct;100(2):1042-3. doi: 10.1104/pp.100.2.1042.
Abstract
Some pulvini of Samanea saman Mehr. labeled with radioactive phosphate show apparent remarkable elevations of the levels of phosphatidic acid and phosphatidylinositol 4,5-bisphosphate. The elevated levels, however, appear to be illusory and to result from rapid initial incorporation of label into these phospholipids relative to others. These results demonstrate the need for caution in interpreting apparent changes in the levels of phosphatidic acid or inositol phospholipids in cultures or plants labeled with radioactive precursors.
摘要
一些 Samanea saman Mehr 的叶枕用放射性磷酸盐标记后，显示出明显的磷脂酸和磷脂酰肌醇 4,5-二磷酸水平的显著升高。然而，这些升高的水平似乎是虚幻的，是由于相对其他磷脂而言，标签快速初始掺入这些磷脂所致。这些结果表明，在用放射性前体标记的培养物或植物中，在解释磷脂酸或肌醇磷脂水平的明显变化时需要谨慎。
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Artifactual Elevation of the Apparent Levels of Phosphatidic Acid and Phosphatidylinositol 4,5-Bisphosphate during Short-Term Labeling of Plant Tissue with Radioactive Precursor.在使用放射性前体对植物组织进行短期标记期间，磷酸脂酸和磷脂酰肌醇 4,5-二磷酸的表观水平会人为升高。
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