Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Avda, San Alberto Magno s/n, E-14071-Córdoba, Spain.
Plant Physiol. 1992 Nov;100(3):1575-9. doi: 10.1104/pp.100.3.1575.
The intracellular localization of the activity and synthesis of three isozymes of NAD(P)(+)-glutamate dehydrogenase from the unicellular green alga Chlamydomonas reinhardtii cw-92 has been established. Isozyme activities have been located within mitochondria by using differential centrifugation techniques and discontinuous Percoll gradient separations. Experiments with protein synthesis inhibitors cycloheximide, rifampicin, chloramphenicol, and actinomycin D, under dark and carbon starvation conditions, revealed that synthesis of the three isozymes was likely to occur in cytosol as precursor proteins that are then transported and processed inside the mitochondria.
已确定单细胞绿藻莱茵衣藻 cw-92 的三种 NAD(P)(+)谷氨酸脱氢酶同工酶的活性和合成的细胞内定位。通过差速离心技术和不连续的 Percoll 梯度分离，将同工酶活性定位于线粒体中。在黑暗和碳饥饿条件下，用蛋白合成抑制剂环己亚胺、利福平、氯霉素和放线菌素 D 进行的实验表明，三种同工酶的合成可能发生在细胞质中，作为前体蛋白，然后在细胞器内运输和加工。
