Institut für Biochemie der Pflanze, Untere Karspüle 2, 3400 Göttingen, Germany.
Plant Physiol. 1992 Dec;100(4):1840-5. doi: 10.1104/pp.100.4.1840.
In leaves of tomato (Lycopersicon esculentum), the synthesis of a light-harvesting complex (LHC) polypeptide of photosystem II and the quinone B (Q(B))-binding protein varies at different time points during the day. In vivo labeling with [(35)S]methionine revealed diurnal oscillations of synthesis of these thylakoid membrane proteins. Both proteins are synthesized at elevated levels right after the transition from darkness to light, a maximum is reached around noon, and decreasing levels were measured during the afternoon and night. In addition, in constant darkness both proteins were also synthesized to varied extents at different diurnal time points. Together, these results indicate that the synthesis of a LHC II and the Q(B)-binding protein is under the control of the circadian clock. This circadian oscillation of LHC II protein synthesis correlates with the very well documented circadian Lhc a/b mRNA accumulation.
在番茄（Lycopersicon esculentum）的叶片中，光系统 II 的捕光复合物（LHC）多肽和醌 B（Q（B））结合蛋白的合成在一天中的不同时间点发生变化。用[（35）S]甲硫氨酸进行体内标记揭示了这些类囊体膜蛋白合成的昼夜振荡。这两种蛋白质在从黑暗到光照的过渡后立即以高水平合成，中午达到最大值，下午和晚上测量到的水平下降。此外，在持续的黑暗中，这两种蛋白质也在不同的昼夜时间点以不同的程度被合成。总之，这些结果表明，LHC II 和 Q（B）结合蛋白的合成受到生物钟的控制。LHC II 蛋白合成的这种昼夜振荡与文献中非常详细的 Lhc a/b mRNA 积累的昼夜振荡相关。
