Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
Plant Physiol. 1991 Nov;97(3):1260-4. doi: 10.1104/pp.97.3.1260.
The levels of abscisic acid (ABA) during embryogenesis in the soybean (Glycine max) cultivar Dare were quantitated. An increase in the quantity of ABA per cotyledon was correlated with a decrease in the chlorophyll a/b binding (Cab) protein gene mRNA population. Soybean cotyledons were cultured in vitro in the presence or absence of ABA. Quantitation of cotyledonary ABA levels and Cab mRNA levels indicated that the application of 5 x 10(-5) molar and 5 x 10(-6) molar exogenous ABA decreased Cab mRNA prevalences. S1 nuclease protection experiments demonstrated that exogenous ABA modulated the level of Cab3 mRNA. These data strongly suggest that one of the developmental regulators of Cab gene expression during soybean embryogeny is the plant hormone, ABA; ABA negatively regulates Cab mRNA accumulation.
对大豆(Glycine max)品种 Dare 在胚胎发生过程中脱落酸(ABA)的水平进行了定量。每枚子叶中 ABA 的含量增加与叶绿素 a/b 结合(Cab)蛋白基因 mRNA 群体减少相关。在有或没有 ABA 的情况下将大豆子叶在体外培养。定量分析子叶 ABA 水平和 Cab mRNA 水平表明,应用 5 x 10(-5) 摩尔和 5 x 10(-6) 摩尔外源 ABA 降低了 Cab mRNA 的流行率。S1 核酸酶保护实验表明,外源 ABA 调节了 Cab3 mRNA 的水平。这些数据强烈表明,ABA 是大豆胚胎发生过程中 Cab 基因表达的发育调节剂之一;ABA 负调控 Cab mRNA 的积累。