Tan Z, Boss W F
Department of Botany, North Carolina State University, Raleigh, North Carolina 27695-7612.
Plant Physiol. 1992 Dec;100(4):2116-20. doi: 10.1104/pp.100.4.2116.
Phosphatidylinositol kinase (PI), phosphatidylinositol monophosphate (PIP) kinase, and diacylglycerol (DAG) kinase activities were detected in the cytoskeletal fraction isolated from microsomes and plasma membranes of carrot (Daucus carota L.) cells grown in suspension culture. The lipid kinase activities were associated with the actin filament fraction (F-actin fraction) isolated from the cytoskeleton. The PI and PIP kinase activity in the F-actin fraction significantly increased after cells were treated with Driselase, a mixture of cell wall-degrading enzymes; however, the DAG kinase activity in the F-actin fraction was unaffected by the Driselase treatment. These data indicate that at least one form of PI, PIP, and DAG kinase preferentially associates with actin filaments and/or actin binding proteins and that cytoskeletal-associated PI and PIP kinase activities can change in response to external stimulation.
在从悬浮培养的胡萝卜（Daucus carota L.）细胞的微粒体和质膜中分离出的细胞骨架部分中检测到磷脂酰肌醇激酶（PI）、磷脂酰肌醇单磷酸（PIP）激酶和二酰基甘油（DAG）激酶活性。脂质激酶活性与从细胞骨架中分离出的肌动蛋白丝部分（F-肌动蛋白部分）相关。在用细胞壁降解酶混合物Driselase处理细胞后，F-肌动蛋白部分中的PI和PIP激酶活性显著增加；然而，Driselase处理对F-肌动蛋白部分中的DAG激酶活性没有影响。这些数据表明，至少一种形式的PI、PIP和DAG激酶优先与肌动蛋白丝和/或肌动蛋白结合蛋白结合，并且细胞骨架相关的PI和PIP激酶活性可以响应外部刺激而发生变化。
