合成并释放促肾上腺皮质激素的大鼠垂体细胞单层培养中的生长

Growth in monolayer culture of rat pituitary cells which synthetize and release acth.

作者信息

Lang R E, Hilwig I, Voigt K H, Fehm H L, Pfeiffer E F

出版信息

Acta Endocrinol (Copenh). 1975 Jul;79(3):421-30. doi: 10.1530/acta.0.0790421.

DOI:10.1530/acta.0.0790421

Abstract

Cultures of rat pituitary gland cells were developed to study biosynthesis and release of ACTH. ACTH measurement was accomplished by radioimmunoassay. ACTH release was observed following stimulation with theophylline and cAMP in a dose-dependent manner. Biosynthesis was demonstrated by incorporation of 3-H-phenylalanine into the hormone, employing a double antibody technique.

摘要

培养大鼠垂体腺细胞以研究促肾上腺皮质激素（ACTH）的生物合成和释放。通过放射免疫测定法完成ACTH的测量。观察到氨茶碱和环磷酸腺苷（cAMP）刺激后ACTH的释放呈剂量依赖性。采用双抗体技术，通过将3 - H - 苯丙氨酸掺入该激素来证明生物合成。