Kirkpatrick J F, Liu I M, Turner J W, Bernoco M
Department of Biological Sciences, Eastern Montana College, Billings 59101.
J Reprod Fertil Suppl. 1991;44:321-5.
Twenty-six free-roaming feral mares were immunized against porcine zonae pellucidae (PZP) between February and May, 1988. Eight sexually mature mares received 2 inoculations 2 weeks apart, and 18 mares received 3 inoculations at intervals of 2 and 4 weeks. Analysis of urinary oestrone conjugates (E1C) and non-specific progesterone metabolites (iPdG) in samples collected in October, 1988, revealed that none of the 18 mares that received 3 and only 1 of the 6 mares that received two inoculations were pregnant, whereas 3 of 6 sham-injected control mares and 5 of 11 untreated mares were pregnant. In February and March, 1989, 14 of the immunized mares that were not pregnant were given a single booster inoculation of PZP. Urinary E1C and iPdG analysis, from samples collected in August and October, 1989, revealed only a single pregnancy among the 14 boosted mares, whereas 33% of mares treated in 1988, but not given a booster inoculation in 1989, and 7 of 16 untreated controls, were pregnant. Foaling was reflected accurately by the urinary steroid metabolite analyses. These results suggest that once recognition of PZP antigen occurs in mares, subsequent annual inoculations will raise antibody titres sufficiently to inhibit fertility.
1988年2月至5月间，对26匹自由放养的野生母马进行了猪透明带（PZP）免疫。8匹性成熟母马每隔2周接种2次，18匹母马每隔2周和4周接种3次。对1988年10月采集的样本中的尿雌酮结合物（E1C）和非特异性孕酮代谢物（iPdG）进行分析，结果显示，接种3次的18匹母马中无一怀孕，接种2次的6匹母马中只有1匹怀孕，而6匹假注射对照母马中有3匹怀孕，11匹未处理母马中有5匹怀孕。1989年2月和3月，对14匹未怀孕的免疫母马进行了一次PZP加强接种。对1989年8月和10月采集的样本进行尿E1C和iPdG分析，结果显示，14匹加强接种的母马中只有1匹怀孕，而1988年接受治疗但1989年未进行加强接种的母马中有33%怀孕，16匹未处理对照中有7匹怀孕。尿类固醇代谢物分析准确反映了产驹情况。这些结果表明，一旦母马识别出PZP抗原，随后每年接种将足以提高抗体滴度以抑制生育能力。
