豚鼠气道内血栓素A2与白三烯在体内的相互作用。

Interaction of thromboxane A2 and leukotrienes in guinea pig airways in vivo.

作者信息

Fujimura M, Bando T, Mizuhashi K, Matsuda T

机构信息

Third Department of Internal Medicine, Kanazawa University, School of Medicine, Japan.

出版信息

Prostaglandins. 1991 Oct;42(4):379-89. doi: 10.1016/0090-6980(91)90086-u.

DOI:10.1016/0090-6980(91)90086-u

PMID:1665570

Abstract

Effects of a thromboxane A2 receptor antagonist (S-1452) on bronchoconstriction induced by inhaled leukotriene C4 and a leukotriene receptor antagonist (AS-35) on bronchoconstriction caused by inhalation of a thromboxane A2 mimetic (STA2) were studied in anesthetized, artificially ventilated guinea pigs in order to examine the interaction of thromboxane A2 and leukotrienes in airways. 0.01-1.0 mu g/ml of leukotriene C4 and 0.1-1.0 mu g/ml of STA 2 inhaled from ultrasonic nebulizer developed for small animals caused dose-dependent increase of pressure at the airway opening (Pao) which is considered to be an index representing bronchial response. Pretreatment of the animals with inhaled S-1452 (0.01, 0.033 mg/ml) significantly reduced the airway responses produced by 0.01,0.033,0.1,0.33 and 1.0 mu g/ml of leukotriene C4 in a dose dependent manner. While pretreatment with inhaled AS-35 (1mg) did not affect the STA2 dose-response curve. These findings suggest that leukotriene C4 activates thromboxane A2 generation while thromboxane A2 does not influence 5-lipoxygenase pathway in the airways.

摘要

为了研究血栓素A2和白三烯在气道中的相互作用，在麻醉、人工通气的豚鼠中研究了血栓素A2受体拮抗剂（S-1452）对吸入白三烯C4诱导的支气管收缩的影响，以及白三烯受体拮抗剂（AS-35）对吸入血栓素A2类似物（STA2）引起的支气管收缩的影响。从为小动物开发的超声雾化器吸入0.01-1.0μg/ml的白三烯C4和0.1-1.0μg/ml的STA2会导致气道开口处压力（Pao）呈剂量依赖性增加，这被认为是代表支气管反应的指标。用吸入的S-1452（0.01、0.033mg/ml）预处理动物可显著降低由0.01、0.033、0.1、0.33和1.0μg/ml白三烯C4产生的气道反应，且呈剂量依赖性。而用吸入的AS-35（1mg）预处理并不影响STA2的剂量反应曲线。这些发现表明，白三烯C4激活血栓素A2的生成，而血栓素A2不影响气道中的5-脂氧合酶途径。