[钾离子、钠离子通道阻滞剂对大鼠黄体细胞孕酮生成的影响]
[Effect of K+, Na+ ionic channel blocker on progesterone production by rat corpus luteal cells].
作者信息
Zhao Y L, Wang J H, Cheng C P
机构信息
Department of Physiology, Harbin Medical University.
出版信息
Sheng Li Xue Bao. 1991 Oct;43(5):508-11.
Two ionic channel blockers, TEA and TTX, were used in the present investigation to test whether blocking of Na+, K+ ion channels would affect the production of progesterone by corpus luteum (CL) cells of rat. Both TEA (10(-3) mol/L) and TTX (10(-3) mol/L) increased progesterone production significantly after treatment. This effect of the blockers could be completely inhibited by tyrosine (Tyr). In addition, Tyr was capable of reversing the combined effect of TEA, TTX and hCG on progesterone secretion. It is suggested that the concentration gradient of Na+ and K+ across the CL cell membrane in experiments mentioned above is implicated in steroidogenesis.
在本研究中使用了两种离子通道阻滞剂，即四乙铵（TEA）和河豚毒素（TTX），以测试阻断Na⁺、K⁺离子通道是否会影响大鼠黄体（CL）细胞孕酮的产生。TEA（10⁻³mol/L）和TTX（10⁻³mol/L）处理后均显著增加了孕酮的产生。酪氨酸（Tyr）可完全抑制这些阻滞剂的这种作用。此外，Tyr能够逆转TEA、TTX和人绒毛膜促性腺激素（hCG）对孕酮分泌的联合作用。提示上述实验中CL细胞膜上Na⁺和K⁺的浓度梯度与类固醇生成有关。
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