Atkinson T G, Allen P J
Department of Botany, University of Wisconsin, Madison, Wisconsin.
Plant Physiol. 1966 Jan;41(1):28-33. doi: 10.1104/pp.41.1.28.
Filter paper, nonabsorbent cotton, and cotton wax were found to be progressively richer sources of germination-stimulatory activity effective in counteracting the self-inhibition of Puccinia graminis var. tritici Erikss. and E. Henn uredospores. The major stimulatory component of cotton wax was purified and partially characterized. It was catalytically effective in stimulating germination and oxygen consumption of uredospores and appeared to be as active as pelargonaldehyde. Unlike most of the previously reported chemical stimulants, however, it was not active across an air gap.Although the active compound was not identified, both the ultraviolet spectrum and the nonionic and solubility properties of the active fractions were consistent with the infrared spectrum which indicated a relatively long-chained. alpha. beta unsaturated carbonyl compound such as a ketone or possibly an ester.The purification procedure involved deionization of ethanolic extracts from cotton or cotton wax on Dowex 50 (H(+)) and Dowex 1 (OH(-)) columns followed by chromatography on neutral alumina using ethylene dichloride as the developing and eluting solvent.
滤纸、脱脂棉和棉蜡被发现是萌发刺激活性的日益丰富的来源，这些活性在对抗小麦条锈菌夏孢子的自我抑制方面有效。棉蜡的主要刺激成分被纯化并进行了部分表征。它在刺激夏孢子萌发和耗氧方面具有催化活性，并且似乎与壬醛一样活跃。然而，与大多数先前报道的化学刺激剂不同，它在气隙中没有活性。尽管活性化合物尚未确定，但活性部分的紫外光谱以及非离子和溶解性特性与红外光谱一致，红外光谱表明是一种相对长链的α,β-不饱和羰基化合物，如酮或可能是酯。纯化过程包括将棉或棉蜡的乙醇提取物在Dowex 50（H⁺）和Dowex 1（OH⁻）柱上进行去离子化，然后使用二氯乙烷作为展开和洗脱溶剂在中性氧化铝上进行色谱分离。
