Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota 55101.
Plant Physiol. 1968 Feb;43(2):201-7. doi: 10.1104/pp.43.2.201.
Malic enzyme was found in both bean rust and cat stem rust uredospores. In bean rust uredospores it was shown to catalyze the formation of pyruvic acid from l-malic acid and to synthesize malic acid from pyruvic acid and CO(2). The malic enzyme from bean rust uredospores was specific for NADP and dependent on manganous ions for activity. The specific activity of the bean rust malic enzyme in crude extracts of ungerminated uredospores was approximately 6 times greater than that found in crude extracts obtained from germinated uredospores. The malic enzyme was also found in extracts obtained from healthy and rust-infected bean leaves. The specific activity of the enzyme was approximately 2 to 5 times greater in partially purified extracts obtained from the infected bean tissue at 6 days after inoculation. The specific activity of the malic enzyme in crude extracts obtained from oat stem rust uredospores was 2 times greater than the specific activity of this enzyme in crude extracts obtained from bean rust uredospores. Phosphoenolpyruvate carboxylase activity could not be demonstrated in crude extracts obtained from the ungerminated uredospores of the bean rust fungus.
苹果酸酶存在于菜豆锈菌和燕麦秆锈菌的夏孢子中。在菜豆锈菌的夏孢子中，该酶被证明能催化 L-苹果酸形成丙酮酸，并能使丙酮酸和 CO2合成苹果酸。菜豆锈菌夏孢子中的苹果酸酶特异性地作用于 NADP，活性依赖于锰离子。未萌发的夏孢子粗提物中菜豆锈菌苹果酸酶的比活约比萌发的夏孢子粗提物中的比活高 6 倍。该酶也存在于健康和感染锈菌的菜豆叶片提取物中。在接种后 6 天从感染的菜豆组织中获得的部分纯化提取物中，酶的比活约高 2 至 5 倍。从燕麦秆锈菌夏孢子粗提物中获得的苹果酸酶的比活比从菜豆锈菌夏孢子粗提物中获得的苹果酸酶的比活高 2 倍。在菜豆锈菌未萌发的夏孢子粗提物中不能证明存在磷酸烯醇丙酮酸羧激酶活性。
