用柱层析法区分吲哚乙酸和柑橘植物生长素。
Differentiation between indoleacetic Acid and the citrus auxin by column chromatography.
机构信息
University of California Citrus Research Center, Riverside.
出版信息
Plant Physiol. 1966 Feb;41(2):208-10. doi: 10.1104/pp.41.2.208.
A gradient elution column chromatography technique and a step-wise technique succeeded in differentiating between IAA and the citrus auxin. IAA was eluted ahead of the citrus auxin in both systems. The highest Avena curvature ever obtained from the citrus auxin occurred after the auxin had passed through the 2 purification techniques and a paper chromatography step. This is probably due to the elimination of inhibitors. Fluorometric assay, Ehrlich's reaction, thin-layer chromatography, and biological assay were used for the detection of IAA or citrus auxin in the column eluates.
梯度洗脱柱层析技术和逐步技术成功地区分了 IAA 和柑橘生长素。在这两种系统中，IAA 都先于柑橘生长素被洗脱出来。从柑橘生长素中获得的最高燕麦弯曲度发生在生长素通过 2 种纯化技术和纸层析步骤之后。这可能是由于抑制剂的消除。荧光测定法、Ehrlich 反应、薄层层析法和生物测定法用于检测柱洗脱液中的 IAA 或柑橘生长素。
Zotero 插件