Wood P M, Bhagavan H N, Crane F L
Department of Biological Sciences, Purdue University, Lafayette, Indiana.
Plant Physiol. 1966 Apr;41(4):633-40. doi: 10.1104/pp.41.4.633.
In order to avoid the problem of an uncoupling effect in stimulation of potential Hill reaction activity during extraction with heptane. Hill reaction activity was measured with uncoupled spinach chloroplasts. The reactions were also run under aerobic conditions. Under these conditions a correction for a 3-(p-chlorophenyl)-1, 1-dimethylurea insensitive, photostimulated O(2) uptake reaction is necessary, especially with extracted chloroplasts. The reaction displayed proper Hill reaction stoichiometry in untreated and restored systems if these corrections were made. Plastoquinone A always restored some activity to the extracted chloroplasts but never restored activity to the original level. Additional activity could be restored by the crude extract. It appears that there is a clear requirement for plastoquinone A in the Hill reaction but the restoration of maximum activity depends upon additional factors. The variability of response to plastoquinone A which has been reported in various laboratories may be determined by the degree of extraction of the other materials.
为避免在庚烷提取过程中刺激潜在希尔反应活性时出现解偶联效应问题，用解偶联的菠菜叶绿体测定希尔反应活性。反应也在有氧条件下进行。在这些条件下，特别是对于提取的叶绿体，有必要对3-(对氯苯基)-1,1-二甲基脲不敏感的光刺激O₂摄取反应进行校正。如果进行了这些校正，反应在未处理和恢复的系统中显示出适当的希尔反应化学计量。质体醌A总能使提取的叶绿体恢复一些活性，但从未恢复到原始水平。粗提取物可恢复额外的活性。似乎在希尔反应中对质体醌A有明确需求，但最大活性的恢复取决于其他因素。不同实验室报道的对质体醌A反应的变异性可能由其他物质的提取程度决定。
