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引用本文的文献

1
Participation of β-carotene in reactivation of PSI of heptane-extracted spinach chloroplasts.β-胡萝卜素参与正己烷抽提菠菜叶绿体 PSI 的再激活。
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2
Three chloroplast membrane models corresponding to different photosynthetic potentialities in the same plant.三种叶绿体膜模型对应于同一植物中不同的光合作用潜能。
Planta. 1980 Jun;149(1):34-43. doi: 10.1007/BF00386224.
3
Role of Galactolipids in Spinach Chloroplast Lamellar Membranes: II. Effects of Galactolipid Depletion on Phosphorylation and Electron Flow.半乳糖脂在菠菜叶绿体片层膜中的作用：II. 半乳糖脂缺失对磷酸化和电子流的影响。
Plant Physiol. 1976 May;57(5):724-9. doi: 10.1104/pp.57.5.724.
4
Acid-bath Phosphorylation in Heptane-extracted Chloroplasts.正丁醇抽提叶绿体中的酸浴磷酸化作用。
Plant Physiol. 1972 Nov;50(5):591-3. doi: 10.1104/pp.50.5.591.

本文引用的文献

1
Isolation of Two Quinones with Coenzyme Q Activity from Alfalfa.从苜蓿中分离出两种具有辅酶Q活性的醌类物质。
Plant Physiol. 1959 Sep;34(5):546-51. doi: 10.1104/pp.34.5.546.
2
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
3
THE REACTIVITY OF A NATURALLY OCCURRING QUINONE (Q-255) IN PHOTOCHEMICAL REACTIONS OF ISOLATED CHLOROPLASTS.一种天然存在的醌（Q - 255）在分离叶绿体光化学反应中的反应活性。
Proc Natl Acad Sci U S A. 1959 Dec;45(12):1696-702. doi: 10.1073/pnas.45.12.1696.
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Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
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Cofactors and rates of photosynthetic phosphorylation by spinach chloroplasts.菠菜叶绿体的辅因子与光合磷酸化速率
J Biol Chem. 1958 Mar;231(1):277-90.
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Beta-carotene, an active component of chloroplasts.β-胡萝卜素，叶绿体的一种活性成分。
Arch Biochem Biophys. 1957 Aug;70(2):382-91. doi: 10.1016/0003-9861(57)90125-x.
7
Inhibition and uncoupling of photophosphorylation in chloroplasts.叶绿体中光合磷酸化的抑制与解偶联作用。
Brookhaven Symp Biol. 1966;19:169-87.
8
Isolation and fatty acid composition of the plant sulfolipid and galactolipids.植物硫脂和半乳糖脂的分离及脂肪酸组成
J Lipid Res. 1964 Jul;5(3):432-4.
9
Photosynthetic reactions with pyridine nucleotide analogs. II. 3-Pyridinealdehyde-diphosphopyridine nucleotide and 3-pyridinealdehyde-deamino-diphosphopyridine nucleotide.吡啶核苷酸类似物的光合反应。II. 3-吡啶甲醛-二磷酸吡啶核苷酸和3-吡啶甲醛-脱氨基-二磷酸吡啶核苷酸。
Arch Biochem Biophys. 1966 Jul;115(1):35-43. doi: 10.1016/s0003-9861(66)81034-2.
10
Electron transport in chloroplasts. 3. The role of plastoquinone C.叶绿体中的电子传递。3. 质体醌C的作用。
J Biol Chem. 1967 Mar 25;242(6):1155-9.

庚烷提取的菠菜叶绿体中光系统I活性的脂质需求

A Lipid Requirement for Photosystem I Activity in Heptane-extracted Spinach Chloroplasts.

作者信息

Brand J, Krogmann D W, Crane F L

机构信息

Department of Biological Sciences and Biochemistry, Purdue University, West Lafayette, Indiana 47907.

出版信息

Plant Physiol. 1971 Jan;47(1):135-8. doi: 10.1104/pp.47.1.135.

DOI:10.1104/pp.47.1.135
PMID:16657568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365826/
Abstract

A lipid requirement for photosystem I activity in Spinacia oleracea chloroplasts has been characterized. The transfer of electrons from tetramethyl-p-phenylenediamine through the chloroplast photosystem to viologen dye was used as an assay of photosystem I activity. Activity is diminished by prolonged heptane extraction and is partially restored by readdition of the extracted lipid. Extracted chloroplasts require plastocyanin for maximal restoration of activity. The effect of lipid extract in restoration is partially replaced by triglycerides containing unsaturated, C(18) fatty acids. Various potential redox carriers which occur naturally in chloroplasts do not substitute for extracted lipid. Galacto-lipids, sulfolipids, and phospholipids are not involved in the restoration of activity.

摘要

已对菠菜叶绿体中光系统I活性的脂质需求进行了表征。利用从四甲基对苯二胺通过叶绿体光系统到紫精染料的电子转移作为光系统I活性的测定方法。通过庚烷长时间萃取会使活性降低，而重新添加萃取的脂质可使活性部分恢复。萃取后的叶绿体需要质体蓝素才能使活性最大程度恢复。脂质提取物在恢复活性方面的作用部分可被含有不饱和C(18)脂肪酸的甘油三酯替代。叶绿体中天然存在的各种潜在氧化还原载体不能替代萃取的脂质。半乳糖脂、硫脂和磷脂不参与活性的恢复。