Brand J, Krogmann D W, Crane F L
Department of Biological Sciences and Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1971 Jan;47(1):135-8. doi: 10.1104/pp.47.1.135.
A lipid requirement for photosystem I activity in Spinacia oleracea chloroplasts has been characterized. The transfer of electrons from tetramethyl-p-phenylenediamine through the chloroplast photosystem to viologen dye was used as an assay of photosystem I activity. Activity is diminished by prolonged heptane extraction and is partially restored by readdition of the extracted lipid. Extracted chloroplasts require plastocyanin for maximal restoration of activity. The effect of lipid extract in restoration is partially replaced by triglycerides containing unsaturated, C(18) fatty acids. Various potential redox carriers which occur naturally in chloroplasts do not substitute for extracted lipid. Galacto-lipids, sulfolipids, and phospholipids are not involved in the restoration of activity.
已对菠菜叶绿体中光系统I活性的脂质需求进行了表征。利用从四甲基对苯二胺通过叶绿体光系统到紫精染料的电子转移作为光系统I活性的测定方法。通过庚烷长时间萃取会使活性降低,而重新添加萃取的脂质可使活性部分恢复。萃取后的叶绿体需要质体蓝素才能使活性最大程度恢复。脂质提取物在恢复活性方面的作用部分可被含有不饱和C(18)脂肪酸的甘油三酯替代。叶绿体中天然存在的各种潜在氧化还原载体不能替代萃取的脂质。半乳糖脂、硫脂和磷脂不参与活性的恢复。