Jones L W, Kok B
Research Institute for Advanced Studies, 1450 South Rolling Road, Baltimore, Maryland.
Plant Physiol. 1966 Jun;41(6):1037-43. doi: 10.1104/pp.41.6.1037.
A study was made of photoinhibition of spinach chloroplast reactions. The kinetics and spectral characteristics of the photoinhibition over a range between 230 and 700 mmu have been examined. The decline of activity due to preillumination was independent of wavelength, and dependent upon the number of quanta applied, not upon the rate of application. The effectiveness spectra of photoinhibition indicate that active ultraviolet light is absorbed by a pigment which is not a normal light absorber for photosynthesis and acts with a high quantum efficiency (> 0.1) for photoinhibition.Active visible light is absorbed by the pigments which sensitize photosynthesis (chlorophyll, carotenoids). A very low quantum efficiency (about 10(-4)) was observed for the photoinhibition with visible light.The action spectrum of the photoinhibition of dye reduction by chloroplasts and lyophylized Anacystis cells indicated that the damage caused by visible light is due to quanta absorbed by photosystem II. However, since system I might not be involved in dye reduction, the spectra may reflect only damage to photosystem II.
对菠菜叶绿体反应的光抑制作用进行了一项研究。已考察了在230至700毫微米范围内光抑制作用的动力学和光谱特性。预照光导致的活性下降与波长无关，而取决于所施加的量子数，而非施加速率。光抑制作用的效能光谱表明，活性紫外光被一种色素吸收，该色素并非光合作用的正常光吸收体，且对光抑制作用具有较高的量子效率（>0.1）。活性可见光被敏化光合作用的色素（叶绿素、类胡萝卜素）吸收。对于可见光引起的光抑制作用，观察到非常低的量子效率（约10⁻⁴）。叶绿体和冻干的鱼腥藻细胞对染料还原的光抑制作用的作用光谱表明，可见光造成的损伤是由于光系统II吸收的量子所致。然而，由于光系统I可能不参与染料还原，这些光谱可能仅反映了对光系统II的损伤。
