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脱乙酰巴卡丁:一种非特异性解偶联光合作用的物质。

Desaspidin: a nonspecific uncoupler of photophosphorylation.

机构信息

Biochemistry Section, Weizmann Institute of Science, Rehovoth, Israel.

出版信息

Plant Physiol. 1966 Sep;41(7):1231-6. doi: 10.1104/pp.41.7.1231.

Abstract

The inhibitory effect of desaspidin is shown to depend on the oxidation-reduction state of the system investigated. The more reducing the conditions, the more desaspidin-inhibited the system and vice versa.The effect of desaspidin on noncyclic photophosphorylation induced by TPN(+), ferricyanide or DPIP is shown to be due to an uncoupling action.The decrease in effectiveness of desaspidin under oxidizing condition is shown to depend upon a photooxidation catalyzed by photosystem I of chloroplasts, which is prevented, but not reversed, by the presence of reducing agents, such as ascorbate + DPIP.These results are interpreted as indicating that, contrary to previous suggestions, desaspidin is a nonspecific uncoupler of all types of photophosphorylation.

摘要

去甲脱乙酰巴卡丁 III 的抑制作用被证明取决于所研究的氧化还原系统的状态。条件越还原,系统受去甲脱乙酰巴卡丁 III 的抑制作用就越大,反之亦然。去甲脱乙酰巴卡丁 III 对 TPN(+)、铁氰化物或 DPIP 诱导的非循环光合磷酸化的影响被证明是由于解偶联作用。在氧化条件下去甲脱乙酰巴卡丁 III 效力降低的现象被证明取决于叶绿体光系统 I 催化的光氧化,还原剂如抗坏血酸+DPIP 的存在可以阻止这种光氧化,但不能使其逆转。这些结果表明,与先前的建议相反,去甲脱乙酰巴卡丁 III 是一种非特异性的解偶联剂,可以解偶联所有类型的光合磷酸化。

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