关于制备菠菜叶绿体中 Hill 反应活性和光合磷酸化活性的研究。
Concerning the Preparation of Chloroplasts Active in Hill and Photosynthetic Phosphorylation Activities from Leaves of Phaseolus vulgaris.
机构信息
Radiation Biology Laboratory, Smithsonian Institution, Washington, D. C.
出版信息
Plant Physiol. 1966 Oct;41(8):1320-2. doi: 10.1104/pp.41.8.1320.
Abstract
When bean plants are cultivated under certain conditions, liberation of cell contents by grinding with mortar and pestle yields chloroplasts of low Hill and photosynthetic phosphorylation activities, while grinding in a high speed blendor yields active chloroplasts. The inactivity of plastids obtained with mortar and pestle is due to the presence of an inactivator in the homogenate. The culture conditions that determine whether bean leaves ground with mortar and pestle contain inactivator were not determined.
摘要
当在某些条件下培养豆科植物时，用研钵和杵研磨会导致叶绿体的希尔系数和光合磷酸化活性降低，而在高速匀浆器中研磨则会产生活性叶绿体。用研钵和杵获得的质体失活是由于匀浆中存在一种失活剂。用研钵和杵研磨的豆科植物叶片是否含有失活剂取决于培养条件，但这些条件尚未确定。
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本文引用的文献
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The Hill Reaction of Red Kidney Bean Chloroplasts.红芸豆叶绿体的希尔反应
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Biochim Biophys Acta. 1960 May 20;40:257-72. doi: 10.1016/0006-3002(60)91350-0.
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