Taylor A O, Zucker M
Department of Plant Pathology and Botany, The Connecticut Agricultural Experiment Station, New Haven, Connecticut.
Plant Physiol. 1966 Oct;41(8):1350-9. doi: 10.1104/pp.41.8.1350.
The active turnover of chlorogenic acid (3-caffeoylquinic acid(3)), a major phenolic component of Xanthium leaves and potato tuber disks, has been demonstrated in these tissues. Pulse-labelling experiments with radioactive l-phenylalanine and trans-cinnamic acid as well as direct feeding experiments with chlorogenic acid-(14)C labelled in the caffeoyl moiety have been employed in the turnover studies. The rate of turnover is calculated to be on the order of 50 to 100 mmumoles per hour per gram fresh weight of tissue.In Xanthium leaves chlorogenic acid is in part converted to an isochlorogenic acid identified by silica gel chromatography as 3,5-dicaffeoylquinic acid. Radioactivity of the caffeoyl moiety of chlorogenic acid is also incorporated into lignin-like insoluble polymers in the leaf. Turnover of chlorogenic acid in tuber tissue is largely accounted for by the incorporation of the caffeoyl moiety into insoluble polymers in the tissue.The significance of chlorogenic acid turnover is discussed in relation to the perception of the photoperiodic stimulus by leaves and to the possible role of chlorogenic acid in lignin synthesis.
绿原酸（3-咖啡酰奎尼酸(3)）是苍耳叶片和马铃薯块茎切片中的主要酚类成分，在这些组织中已证实其存在活跃的周转。周转研究采用了以放射性L-苯丙氨酸和反式肉桂酸进行脉冲标记实验，以及用咖啡酰部分标记有(14)C的绿原酸进行直接饲喂实验。周转速率经计算约为每克组织鲜重每小时50至100微摩尔。在苍耳叶片中，绿原酸部分转化为一种异绿原酸，通过硅胶色谱法鉴定为3,5-二咖啡酰奎尼酸。绿原酸咖啡酰部分的放射性也掺入叶片中类似木质素的不溶性聚合物中。块茎组织中绿原酸的周转主要是由于咖啡酰部分掺入组织中的不溶性聚合物。本文讨论了绿原酸周转的意义，涉及叶片对光周期刺激的感知以及绿原酸在木质素合成中可能的作用。
