Department of Plant Sciences, Texas A&M University, College Station, Texas.
Plant Physiol. 1966 Nov;41(9):1513-9. doi: 10.1104/pp.41.9.1513.
The potential role of indoleactic acid (IAA)-oxidase as an in vivo abscission regulating system in the cotton (Gossypium hirsutum L.) cotyledonary explant was investigated. Phenols (usually monophenols), which are cofactors of cotton IAA-oxidase in vitro, accelerated abscission. Phenols (usually orthodihydroxyphenols), which inhibit cotton IAA-oxidase in vitro, inhibited abscission. Inhibition or stimulation of abscission was accomplished by phenols both with and without IAA. Results were similar when treatments were applied as lanolin pastes to the cut petiole ends or as solutions in which explants were submerged. An abscission accelerating phenol stimulated the decarboxylation of IAA-1-(14)C by explants and an abscission inhibiting phenol inhibited the decarboxylation of IAA-1-(14)C.The mechanism of abscission regulation by the phenolic compounds was concluded to involve auxin destruction via IAA-oxidase.In addition to the direct relationship of this study to abscission, the results support the more general hypothesis that IAA-oxidase acts in vivo to regulate auxin levels.
我们研究了吲哚乙酸(IAA)-氧化酶在棉花(Gossypium hirsutum L.)子叶外植体的活体离层调控系统中的潜在作用。在体外,酚类物质(通常为单酚类)是棉花 IAA-氧化酶的辅因子,可加速离层的发生。在体外,抑制棉花 IAA-氧化酶的酚类物质(通常为邻二羟基酚类)抑制离层的发生。酚类物质无论是单独使用还是与 IAA 一起使用,都能抑制或刺激离层的发生。当将酚类物质作为羊毛脂糊剂涂抹在切断的叶柄末端或作为外植体浸没的溶液进行处理时,结果相似。一种促进离层的酚类物质刺激外植体中 IAA-1-(14)C 的脱羧作用,而一种抑制离层的酚类物质抑制 IAA-1-(14)C 的脱羧作用。酚类化合物通过 IAA-氧化酶调节离层的机制被归结为生长素的破坏。除了本研究与离层的直接关系外,这些结果还支持了更普遍的假说,即 IAA-氧化酶在体内起作用以调节生长素水平。