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马铃薯块茎先前储存情况对其组织及线粒体组分的耐盐性和染料还原能力的影响

Salt hardiness and dye reduction by potato tissue and mitochondrial fractions as influenced by previous storage of the tubers.

作者信息

Craft C C

机构信息

Agricultural Research Service, United States Department of Agriculture, Pomona, California 91769.

出版信息

Plant Physiol. 1966 Dec;41(10):1662-6. doi: 10.1104/pp.41.10.1662.

Abstract

Oxygen uptake and tetrazolium reduction occurred at higher rates in discs from potato tubers (Solanum tuberosum L.) stored at 0 degrees than in discs from tubers stored at 12.8 degrees . Tetrazolium reduction was at a higher rate in mitochondrial fractions from tubers stored at 0 degrees than in mitochondrial fractions from tubers stored at 12.8 degrees . These physiological activities were more resistant to hypertonic KCl treatments in tissue and mitochondrial fractions from tubers stored at 0 degrees than in tissue and mitochondrial fractions from tubers stored at 12.8 degrees . Inhibition of O(2) uptake and tetrazolium reduction progressively increased with increasing concentrations of KCl for tissue and mitochondrial fractions from tubers stored at 0 and 12.8 degrees , but inhibition was more severe and occurred at lower concentrations of KCl for the material from tubers stored at 12.8 degrees . Tissue from tubers stored at 0 degrees was at the same time more sensitive to hypotonic solutions and more resistant to hypertonic solutions than corresponding tissue from tubers stored at 12.8 degrees . Adaptive changes brought on in the tubers by the stress of cold storage were demonstrated in the discs and mitochondrial fractions prepared from cold-stored tubers.

摘要

在0摄氏度下储存的马铃薯块茎(Solanum tuberosum L.)切片中,氧气摄取和四氮唑还原的速率高于在12.8摄氏度下储存的块茎切片。在0摄氏度下储存的块茎线粒体部分中,四氮唑还原速率高于在12.8摄氏度下储存的块茎线粒体部分。与在12.8摄氏度下储存的块茎的组织和线粒体部分相比,在0摄氏度下储存的块茎的组织和线粒体部分中的这些生理活性对高渗KCl处理更具抗性。对于在0摄氏度和12.8摄氏度下储存的块茎的组织和线粒体部分,随着KCl浓度的增加,O₂摄取和四氮唑还原的抑制作用逐渐增强,但对于在12.8摄氏度下储存的块茎的材料,抑制作用更严重且在较低的KCl浓度下就会出现。与在12.8摄氏度下储存的块茎的相应组织相比,在0摄氏度下储存的块茎的组织对低渗溶液更敏感,而对高渗溶液更具抗性。从冷藏块茎制备的切片和线粒体部分证明了冷藏胁迫给块茎带来的适应性变化。

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