紫花苜蓿根中的可溶性蛋白质与抗寒性的关系

Gerloff E D, Stahmann M A, Smith D

Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 58706.

Plant Physiol. 1967 Jul;42(7):895-9. doi: 10.1104/pp.42.7.895.

Soluble proteins extracted from alfalfa roots of hardy and nonhardy varieties were studied in relation to cold hardiness with polyacrylamide gel electrophoresis and quantitative enzyme analysis. Soluble protein content of alfalfa roots increased during hardening in all varieties. Two new isoenzymes with peroxidase activities were found in the fully hardened samples but no large shifts in the electrophoretic pattern were detected with polyacrylamide gel electrophoresis. Peroxidase and catalase activities increased during hardening in all varieties, but only small differences among hardy and nonhardy varieties were detectable. The studies indicated that protein metabolism was altered during the hardening process.

利用聚丙烯酰胺凝胶电泳和定量酶分析方法，对耐寒和不耐寒品种苜蓿根中提取的可溶性蛋白质与抗寒性的关系进行了研究。在所有品种中，苜蓿根的可溶性蛋白质含量在硬化过程中均有所增加。在完全硬化的样品中发现了两种具有过氧化物酶活性的新同工酶，但聚丙烯酰胺凝胶电泳未检测到电泳图谱有大的变化。在所有品种中，过氧化物酶和过氧化氢酶活性在硬化过程中均增加，但耐寒和不耐寒品种之间仅检测到微小差异。研究表明，在硬化过程中蛋白质代谢发生了改变。