植物亲缘关系的 DNA-琼脂技术测定。
Relatedness Among Plants as Measured by the DNA-Agar Technique.
机构信息
Department of Terrestrial Magnetism, Carnegie Institution of Washington, Washington, D. C. 20015.
出版信息
Plant Physiol. 1967 Jul;42(7):959-67. doi: 10.1104/pp.42.7.959.
Abstract
An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosae and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.
摘要
描述了一种改进的植物 DNA 提取方法。报道了几种豆科植物以及大麦、小麦和黑麦的 DNA-DNA 杂交定量种间比较。在所测试的单子叶植物和双子叶植物之间，DNA 多核苷酸序列的同源性最多约为 10%，而在一个科内则观察到 20%至 90%的同源性。使用富含重复多核苷酸序列的 DNA 部分进行比较的物种通常比使用整个 DNA 时更为密切相关。DNA-DNA 杂交可能在植物的系统发生和进化研究中有用，也可能作为种间可育性的一种可能的筛选程序。
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