Chisholm M D, Wetter L R
Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatohewan, Canada.
Plant Physiol. 1967 Dec;42(12):1726-30. doi: 10.1104/pp.42.12.1726.
The incorporation of the radioactivity from acetate-1-(14)C, acetate-2-(14)C, dl-methionine-1-(14)C, dl-methionine-2-(14)C, dl-methionine-3,4-(14)C, dl-homomethionine-2-(14)C, dl-allyl-glycine-2-(14)C, and dl-2-amino-5-hydroxyvalerate-2-(14)C into the aglycones of progoitrin, gluconapin, and glucobrassicanapin of maturing rape plants (Brassica campestris L.) was investigated. Radioactivity from dl-methionine-2-(14)C, dl-methionine-3,4-(14)C, dl-homomethionine-2-(14)C, and acetate-2-(14)C were incorporated into the 3 major thioglucosides. The other organic compounds were poorly incorporated except for dl-allylglycine-2-(14)C into glucobrassicanapin. The results obtained suggest that the rape plant can synthesize amino acids by the condensation of acetate (as acetyl CoA) to alpha-keto acids to yield a homologue of the original amino acid. These newly formed amino acids are then employed to synthesize the 3 major thioglucosides.
研究了成熟油菜植株（甘蓝型油菜）中，来自乙酸 - 1 -（¹⁴C）、乙酸 - 2 -（¹⁴C）、dl - 蛋氨酸 - 1 -（¹⁴C）、dl - 蛋氨酸 - 2 -（¹⁴C）、dl - 蛋氨酸 - 3,4 -（¹⁴C）、dl - 高蛋氨酸 - 2 -（¹⁴C）、dl - 烯丙基甘氨酸 - 2 -（¹⁴C）和dl - 2 - 氨基 - 5 - 羟基戊酸 - 2 -（¹⁴C）的放射性掺入前甲状腺素原、葡糖芥苷和葡糖芸苔素的苷元中的情况。来自dl - 蛋氨酸 - 2 -（¹⁴C）、dl - 蛋氨酸 - 3,4 -（¹⁴C）、dl - 高蛋氨酸 - 2 -（¹⁴C）和乙酸 - 2 -（¹⁴C）的放射性被掺入到3种主要硫代葡萄糖苷中。除了dl - 烯丙基甘氨酸 - 2 -（¹⁴C）掺入葡糖芸苔素外，其他有机化合物的掺入情况较差。所得结果表明，油菜植株可通过乙酸（作为乙酰辅酶A）与α - 酮酸缩合来合成氨基酸，从而产生原始氨基酸的同系物。然后利用这些新形成的氨基酸来合成3种主要硫代葡萄糖苷。
