Red River Valley Potato Processing Laboratory , East Grand Forks, Minnesota 56721.
Plant Physiol. 1967 Dec;42(12):1780-6. doi: 10.1104/pp.42.12.1780.
Invertase inhibitor was extracted from potato tubers and purified nearly 1000-fold. The purification procedure involved precipitation at pH 4.0, fractionation with ammonium sulfate, adsorption on alumina Cgamma gel, and gel filtration on Sephadex G-100 and DEAE-Sephadex A-50. The product obtained was homogeneous to electrophoresis on polyacrylamide gel. Exclusion chromatography on Sephadex G-100 indicated a molecular weight of about 17,000. The inhibitor did not inhibit yeast, Neurospora, and several plant invertases. It completely inhibited potato tuber invertase and a number of other plant invertases. Some plant invertases were partially inhibited.
从土豆块茎中提取并纯化了近 1000 倍的转化酶抑制剂。该纯化程序包括在 pH4.0 时沉淀、硫酸铵分级、氧化铝 Cgamma 凝胶吸附以及葡聚糖凝胶 G-100 和 DEAE-葡聚糖 A-50 的凝胶过滤。所得产物在聚丙烯酰胺凝胶电泳上均一。排阻色谱法在 Sephadex G-100 上表明分子量约为 17000。该抑制剂不抑制酵母、蕈类和几种植物转化酶。它完全抑制土豆块茎转化酶和一些其他植物转化酶。一些植物转化酶受到部分抑制。
