Earnshaw M J, Truelove B
Department of Botany, The University, Manchester 13, England.
Plant Physiol. 1968 Jan;43(1):121-9. doi: 10.1104/pp.43.1.121.
Isolated Phaseolus mitochondria will swell spontaneously in buffered KCl and contract with an oxidizable substrate or ATP + Mg(2+). The conditions under which the mitochondria are swollen affect subsequent contraction, substrate oxidation and ion accumulation, but not their oxidative phosphorylation ability. Bovine serum albumin reduces the rate of swelling and promotes substrate oxidation, contraction and ion accumulation. Swelling of these mitochondria is associated with the release of malic dehydrogenase and a loss of membrane integrity. The beneficial effects of bovine serum albumin in preserving the energy linked functions of Phaseolus mitochondria is discussed.
分离出的菜豆线粒体在缓冲的氯化钾溶液中会自发膨胀，并在可氧化底物或ATP + Mg(2+)存在时收缩。线粒体膨胀的条件会影响随后的收缩、底物氧化和离子积累，但不影响其氧化磷酸化能力。牛血清白蛋白可降低膨胀速率，并促进底物氧化、收缩和离子积累。这些线粒体的膨胀与苹果酸脱氢酶的释放和膜完整性的丧失有关。本文讨论了牛血清白蛋白在维持菜豆线粒体能量相关功能方面的有益作用。
