Dickinson D B, Cochran D
Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
Plant Physiol. 1968 Mar;43(3):411-6. doi: 10.1104/pp.43.3.411.
Five percent dimethyl sulfoxide (DMSO) completely inhibited tube initiation, stopped tube growth and suppressed the high respiration associated with tube growth of lily pollen. The effect of DMSO on respiration was indirect because uncoupling concentrations of 2,4-dinitrophenol abolished the inhibition of respiration. Five percent DMSO did not inhibit rapid starch synthesis during the first 30 minutes of incubation, nor did DMSO inhibit the period of high respiration associated with rapid starch synthesis. DMSO did not cause permanent damage to the cells since normal pollen tube growth occurred after its removal. Dimethyl sulfoxide is not a general inhibitor of pollen metabolism, but it may be a specific inhibitor of a process required for tube growth.
5%的二甲基亚砜(DMSO)完全抑制了花粉管起始,使花粉管生长停止,并抑制了与百合花粉管生长相关的高呼吸作用。DMSO对呼吸作用的影响是间接性的,因为解偶联浓度的2,4-二硝基苯酚消除了对呼吸作用的抑制。5%的DMSO在孵育的前30分钟内不抑制淀粉的快速合成,也不抑制与淀粉快速合成相关的高呼吸期。DMSO不会对细胞造成永久性损伤,因为去除DMSO后花粉管能正常生长。二甲基亚砜不是花粉代谢的一般抑制剂,但它可能是花粉管生长所需过程的特异性抑制剂。