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植物叶和茎蛋白质。I. 基本的、水溶性部分的提取和电泳分离。

Plant leaf and stem proteins. I. Extraction and electrophoretic separation of the basic, water-soluble fraction.

机构信息

Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.

出版信息

Plant Physiol. 1968 Apr;43(4):578-82. doi: 10.1104/pp.43.4.578.

DOI:10.1104/pp.43.4.578
PMID:16656809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1086890/
Abstract

Simplified but highly reproducible extraction and electrophoretic procedures have been developed for plant stem and leaf proteins using recent chemical and technical advances. The method is applied to the separation of the basic, water-soluble proteins found in stem and leaf tissues of 3 Dianthus clones. While most of the highly reproducible protein bands appear in all 3 selections, and many are also common to both leaf and stem tissue, others are characteristic for the variety or tissue.

摘要

采用最新的化学和技术进展，为植物茎和叶蛋白质开发了简化但高度可重现的提取和电泳程序。该方法应用于 3 个石竹克隆体的茎和叶组织中发现的碱性、水溶性蛋白质的分离。虽然大多数高度可重现的蛋白质条带出现在所有 3 个选择中，并且许多也存在于叶和茎组织中，但其他条带则是该品种或组织所特有的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/c16574996abb/plntphys00483-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/baa7058f0e1a/plntphys00483-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/5d6b70ea5a44/plntphys00483-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/c16574996abb/plntphys00483-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/baa7058f0e1a/plntphys00483-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/5d6b70ea5a44/plntphys00483-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94bc/1086890/c16574996abb/plntphys00483-0099-b.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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