Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.
Plant Physiol. 1968 Apr;43(4):578-82. doi: 10.1104/pp.43.4.578.
Simplified but highly reproducible extraction and electrophoretic procedures have been developed for plant stem and leaf proteins using recent chemical and technical advances. The method is applied to the separation of the basic, water-soluble proteins found in stem and leaf tissues of 3 Dianthus clones. While most of the highly reproducible protein bands appear in all 3 selections, and many are also common to both leaf and stem tissue, others are characteristic for the variety or tissue.
采用最新的化学和技术进展,为植物茎和叶蛋白质开发了简化但高度可重现的提取和电泳程序。该方法应用于 3 个石竹克隆体的茎和叶组织中发现的碱性、水溶性蛋白质的分离。虽然大多数高度可重现的蛋白质条带出现在所有 3 个选择中,并且许多也存在于叶和茎组织中,但其他条带则是该品种或组织所特有的。