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醛缩酶在光合作用中的作用。II. 光合生物中醛缩酶类型的证明。

Role of Aldolase in Photosynthesis. II Demonstration of Aldolase Types in Photosynthetic Organisms.

作者信息

Willard J M, Gibbs M

机构信息

Department of Biochemistry, Cornell University, Waltham, Massachusetts 02154.

出版信息

Plant Physiol. 1968 May;43(5):793-8. doi: 10.1104/pp.43.5.793.

Abstract

Spinach leaves and photoautotrophically grown Euglena and Chlorella possess fructose 1,6-diphosphate aldolases inhibited by p-chloromercuribenzoate but insensitive to K(+) or ethylenediamine tetraacetate (Type I). Dark grown Euglena and Chlorella have aldolases inhibited by p-chloromercuribenzoate and ethylenediamine tetraacetate but stimulated by K(+) (Type II). The red alga, Chondrus, and the golden-brown alga, Ochromonas, appear to possess both types. Bean, pea, and spinach seeds and the leaves and cotyledons of etiolated bean seedlings contain a p-chloromercuribenzoate insensitive, apparently non-sulfhydryl variant of Type I. Sensitivity of leaf aldolase to p-chloromercuribenzoate occurs in etiolated bean seedlings only after an extended period of illumination. Type II aldolase activity in cell-free extracts of 4 blue-green algae has been demonstrated.

摘要

菠菜叶以及光合自养生长的眼虫和小球藻含有果糖1,6 - 二磷酸醛缩酶,该酶受对氯汞苯甲酸抑制,但对钾离子或乙二胺四乙酸不敏感(I型)。黑暗中生长的眼虫和小球藻的醛缩酶受对氯汞苯甲酸和乙二胺四乙酸抑制,但受钾离子刺激(II型)。红藻角叉菜和金褐藻赭球藻似乎同时拥有这两种类型。豆类、豌豆和菠菜种子以及黄化豆苗的叶子和子叶含有一种对氯汞苯甲酸不敏感、显然不含巯基的I型变体。只有在长时间光照后,黄化豆苗叶片醛缩酶才会对氯汞苯甲酸敏感。已经证明4种蓝藻的无细胞提取物中存在II型醛缩酶活性。

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Aldolase in blue-green algae.蓝绿藻中的醛缩酶。
Nature. 1965 Apr 10;206(980):193-5. doi: 10.1038/206193a0.

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