Department of Botanical Sciences and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Plant Physiol. 1968 Jul;43(7):1011-6. doi: 10.1104/pp.43.7.1011.
A burst of ribosomal RNA synthesis is induced in potato tissue by slicing, and continues at a decreasing rate for about 12 hours. Ribosomal RNA synthesis in potato discs is sensitive to puromycin, in contrast to non-ribosomal RNA synthesis. Thus, the influence of puromycin on total RNA synthesis is significant only during the first 12 hours following slicing. The function of RNA made after 12 hours in a puromycin-insensitive manner is unknown. However, it is apparently unrelated to protein synthesis, since it has been shown that total inhibition of RNA synthesis by addition of actinomycin D to potato tissue after 12 hours of aging has no effect upon protein synthesis during the ensuing 12 hours.
切割会诱导马铃薯组织中核糖体 RNA 的合成爆发,并以大约 12 小时的递减速率继续进行。与非核糖体 RNA 合成相反,马铃薯切片中的核糖体 RNA 合成对嘌呤霉素敏感。因此,嘌呤霉素对总 RNA 合成的影响仅在切割后 12 小时内显著。以嘌呤霉素不敏感的方式在 12 小时后产生的 RNA 的功能尚不清楚。然而,它显然与蛋白质合成无关,因为已经表明,在老化 12 小时后向马铃薯组织中添加放线菌素 D 以完全抑制 RNA 合成,对随后 12 小时内的蛋白质合成没有影响。