Facultad de Farmacia y Bioquimica, Junin 956.
Plant Physiol. 1968 Jul;43(7):1063-8. doi: 10.1104/pp.43.7.1063.
Sucrose and sucrose 6-phosphate synthetase were isolated from potato tubers, partially purified and their properties studied. The sucrose synthetase showed optimum activity at 45 degrees and was inhibited competitively by ADP and some phenolic glucosides. The Ki's for these inhibitors were determined. Mg(2+) was found to activate this enzyme. Activity toward UDP-glucose or ADP-glucose formation was measured. The optimum conditions for sucrose and UDP-glucose formation were found to differ. The specificity for the glucosyl donor and acceptor were determined.The optimum conditions for sucrose 6-phosphate synthetase activity were studied. This enzyme was not inhibited by either ADP or phenolic glucosides; UDP-glucose was the only glucosyl donor for sucrose 6-phosphate formation.
从马铃薯块茎中分离出蔗糖和蔗糖 6-磷酸合成酶,对其进行了部分纯化并研究了其性质。蔗糖合成酶在 45°C 时表现出最佳活性,并被 ADP 和一些酚类糖苷竞争性抑制。测定了这些抑制剂的 Ki 值。发现 Mg2+ 能激活该酶。测量了 UDP-葡萄糖或 ADP-葡萄糖形成的活性。发现蔗糖和 UDP-葡萄糖形成的最佳条件不同。确定了葡萄糖供体和受体的特异性。研究了蔗糖 6-磷酸合成酶活性的最佳条件。该酶不受 ADP 或酚类糖苷的抑制;UDP-葡萄糖是合成蔗糖 6-磷酸的唯一葡萄糖供体。