Trip P, Gorham P R
Division of Biosciences, National Research Council, Ottawa, Ontario, Canada.
Plant Physiol. 1968 Nov;43(11):1845-9. doi: 10.1104/pp.43.11.1845.
When (14)C-sugar and THO were simultaneously introduced through a cut side vein or flap of a squash leaf (Cucurbita melopepo, Bailey cv. torticollis) concurrent translocation of (14)C-sugars, T-photosynthates and THO with parallel, almost flat, gradients was observed in the petiole for periods of 1 to 3 hr. Parallel translocation gradients were not observed when (14)C was introduced as (14)CO(2) and T by painting a leaf with THO. Autoradiography of frozen sections to locate the tissues in which THO was moving was unsuccessful. Steam-girdling blocked the movement of (14)C and T when (14)C-glucose and THO were introduced simultaneously by the flap-feeding technique. If THO moved as liquid water in the phloem along with the (14)C-sugars, as blockage by steam girdling suggests, then solution flow of sugar cannot be excluded as a mechanism of translocation.
当通过南瓜叶（西葫芦，贝利品种“ torticollis”）的切口侧静脉或叶瓣同时引入¹⁴C-糖和THO时，在1至3小时内，在叶柄中观察到¹⁴C-糖、T-光合产物和THO以平行且几乎平坦的梯度同时转运。当通过用THO涂抹叶片以¹⁴CO₂形式引入¹⁴C并引入T时，未观察到平行的转运梯度。通过对冷冻切片进行放射自显影来定位THO移动的组织未成功。当通过叶瓣饲喂技术同时引入¹⁴C-葡萄糖和THO时，蒸汽环割阻断了¹⁴C和T的移动。如果正如蒸汽环割所表明的那样，THO作为液态水与¹⁴C-糖一起在韧皮部中移动，那么就不能排除糖溶液流动作为一种转运机制。
