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化学固定的鱼腥藻、蛋白核小球藻和紫球藻的光合作用光反应。

Photosynthetic Light Reactions in Chemically Fixed Anacystis nidulans, Chlorella pyrenoidosa, and Porphyridium cruentum.

机构信息

Department of Botany, University of California, Berkeley, California 94720.

出版信息

Plant Physiol. 1969 Apr;44(4):535-9. doi: 10.1104/pp.44.4.535.

DOI:10.1104/pp.44.4.535
PMID:16657097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC396122/
Abstract

The photochemical activities of various species of unicellular algae (Anacystis nidulans, Chlorella pyrenoidosa, and Porphyridium cruentum) were studied following chemical fixation. Fixation with formaldehyde and glutaraldehyde yielded cells which retained their ability to perform photosystem I and photosystem II reactions. The photochemical efficiencies of some fixed algae are as great as those of unfixed spinach chloroplasts. Fixed algae containing accessory pigments appear to be useful models for further studies of the light reactions of photosynthesis.

摘要

研究了各种单细胞藻类(鱼腥藻、蛋白核小球藻和紫球藻)的光化学活性,这些藻类在化学固定后进行研究。用甲醛和戊二醛固定后,细胞保留了进行光合作用 I 和光合作用 II 反应的能力。一些固定藻类的光化学效率与未固定的菠菜叶绿体相当。含有辅助色素的固定藻类似乎是进一步研究光合作用光反应的有用模型。

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引用本文的文献

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Photosynthetic light reactions in chemically fixed spinach thylakoids.化学固定菠菜类囊体中的光合光反应。
Plant Physiol. 1969 Apr;44(4):544-6. doi: 10.1104/pp.44.4.544.
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Plant Physiol. 1969 Apr;44(4):540-3. doi: 10.1104/pp.44.4.540.
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The effect of glutaraldehyde on light-induced H+ changes, electron transport, and phosphorylation in pea chloroplasts.戊二醛对豌豆叶绿体中光诱导的H⁺变化、电子传递及磷酸化作用的影响。
J Bioenerg. 1970 Oct;1(4):405-12. doi: 10.1007/BF01654577.
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Physiology and cytological chemistry blue-green algae.蓝藻的生理学与细胞化学
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Fixation of phycobiliproteins to photosynthetic membranes by glutaraldehyde.通过戊二醛将藻胆蛋白固定到光合膜上。
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本文引用的文献

1
Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum.来自蓝绿藻 luridum 的裂解原生质体和颗粒制剂的光合反应。
Plant Physiol. 1967 Oct;42(10):1447-56. doi: 10.1104/pp.42.10.1447.
2
Uncouplers of Spinach Chloroplast Photosynthetic Phosphorylation.菠菜叶绿体光合磷酸化的解偶联剂。
Plant Physiol. 1959 May;34(3):272-7. doi: 10.1104/pp.34.3.272.
3
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
Plant Physiol. 1949 Jan;24(1):1-15. doi: 10.1104/pp.24.1.1.
4
HILL ACTIVITY IN CELL-FREE PREPARATIONS OF A BLUE-GREEN ALGA.蓝绿藻无细胞制剂中的希尔活性
Biochim Biophys Acta. 1964 Jul 29;88:11-9. doi: 10.1016/0926-6577(64)90150-0.
5
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.细胞化学与电子显微镜术。醛类固定对细胞超微结构及酶活性的保存作用。
J Cell Biol. 1963 Apr;17(1):19-58. doi: 10.1083/jcb.17.1.19.
6
Cofactors and rates of photosynthetic phosphorylation by spinach chloroplasts.菠菜叶绿体的辅因子与光合磷酸化速率
J Biol Chem. 1958 Mar;231(1):277-90.
7
Hill reaction rates and chloroplast fragment size.希尔反应速率与叶绿体片段大小。
Biochim Biophys Acta. 1965 Nov 29;109(2):372-81. doi: 10.1016/0926-6585(65)90164-0.
8
The reducing power generated in photoact I of photosynthesis.光合作用光反应I中产生的还原力。
Biochim Biophys Acta. 1965 Nov 29;109(2):347-56. doi: 10.1016/0926-6585(65)90162-7.
9
Purification and quantitation of glutaraldehyde and its effect on several enzyme activities in skeletal muscle.戊二醛的纯化与定量及其对骨骼肌中几种酶活性的影响。
J Histochem Cytochem. 1967 Aug;15(11):652-61. doi: 10.1177/15.11.652.
10
The Hill reaction of chloroplasts isolated from glutaraldehyde-fixed spinach leaves.从戊二醛固定的菠菜叶片中分离出的叶绿体的希尔反应。
Proc Natl Acad Sci U S A. 1966 May;55(5):1056-62. doi: 10.1073/pnas.55.5.1056.