Veen H, Jacobs W P
Department of Biology, Princeton University, Princeton, New Jersey 08540.
Plant Physiol. 1969 Sep;44(9):1277-84. doi: 10.1104/pp.44.9.1277.
To see if polar movement was typical of growth-regulators other than auxins, the movement of adenine-8-(14)C and of kinetin-8-(14)C was studied in segments cut from petioles of increasing age. No polarity was found. In time-course experiments lasting 24 hr, kinetin showed a progressive increase of radioactivity in receiver blocks, while adenine showed a maximum at 8 hr with a decline thereafter. More kinetin moved through older segments than through younger ones. There was no difference in net loss as far as the position of the donor block is concerned. However, the loss of radioactivity from adenine donor blocks was much higher than the loss of radioactivity from kinetin donor blocks.The radioactivity in receiver blocks after 24 hr treatment with kinetin-(14)C was still with kinetin, judging by location on chromatograms. By the same criterion, adenine and a smaller amount of some other compound were in receiver blocks after a 6 hr transport with adenine-(14)C in the donors. By contrast, more zones of radioactivity were extracted from petiole segments to which kinetin or adenine had been added. For both purine derivatives the original compound represented no more than 20% of the total radioactivity extracted from the tissue after a transport period of 24 hr.
为了探究极性运输是否是生长素以外其他生长调节剂的典型特征，对取自不同年龄叶柄切段中8-(14)C标记的腺嘌呤和8-(14)C标记的激动素的运输进行了研究。未发现极性。在持续24小时的时间进程实验中，激动素在接收块中的放射性呈逐渐增加趋势，而腺嘌呤在8小时时达到最大值，之后下降。与较年轻的切段相比，更多的激动素通过较老的切段运输。就供体块的位置而言，净损失没有差异。然而，腺嘌呤供体块的放射性损失远高于激动素供体块的放射性损失。用(14)C标记的激动素处理24小时后，根据色谱图上的位置判断，接收块中的放射性物质仍为激动素。按照同样的标准，在供体中用(14)C标记的腺嘌呤运输6小时后，接收块中有腺嘌呤和少量其他化合物。相比之下，从添加了激动素或腺嘌呤的叶柄切段中提取出更多的放射性区域。对于这两种嘌呤衍生物，在24小时的运输期后，原始化合物在从组织中提取的总放射性中所占比例不超过20%。
