在萌发的水稻幼苗中诱导硝酸还原酶和优先同化铵。
The induction of nitrate reductase and the preferential assimilation of ammonium in germinating rice seedlings.
机构信息
School of Bioloogical Sciences, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
Plant Physiol. 1969 Nov;44(11):1650-5. doi: 10.1104/pp.44.11.1650.
Abstract
Nitrate reduotase is induced by nitrate in excised embryos and germinating intact seedlings of rice (Oryza sativa L.). The enzyme is induced 24 hr after imbibition. The rate of enzyme formation increases with the age of seedlings. There is a lag period of 30 to 40 min between the addition of substrate and the formation of nitrate reductase. Formation of the enzyme is promoted by the presence of ammonium. Chloramphenicol, actinomycin D and cycloheximide effectively inhibit the formation of nitrate reductase.Rice seedlings can assimilate nitrate from the beginning of germination. However, the utilization of nitrate is completely suppressed by the presence of ammonium. As soon as ammonium is depleted from the medium, nitrate utilization is resumed. Ammonium inhibits the first step of nitrate reduction, i.e., NO(-) (3) --> NO(-) (2), but does not inhibit the assimilation of nitrite. This provides an example of feedback inhibition in higher plants.
摘要
硝酸还原酶可被硝酸盐诱导，在离体胚胎和萌发完整水稻幼苗（Oryza sativa L.）中均有表达。该酶在吸胀后 24 小时被诱导产生。酶的形成速度随幼苗年龄的增加而增加。在添加底物和形成硝酸还原酶之间存在 30 至 40 分钟的滞后期。铵的存在促进了酶的形成。氯霉素、放线菌素 D 和环己酰亚胺能有效抑制硝酸还原酶的形成。水稻幼苗在萌发开始时就能同化硝酸盐。然而，铵的存在完全抑制了硝酸盐的利用。一旦培养基中的铵耗尽，硝酸盐的利用就会恢复。铵抑制硝酸还原的第一步，即 NO(-) (3) --> NO(-) (2)，但不抑制亚硝酸盐的同化。这为高等植物中的反馈抑制提供了一个例子。
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本文引用的文献
1
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Science. 1964 Aug 14;145(3633):674-9. doi: 10.1126/science.145.3633.674.
2
Substrate induction of nitrate reductase in barley aleurone layers.大麦糊粉层中硝酸盐还原酶的底物诱导作用。
Plant Physiol. 1969 Jan;44(1):85-8. doi: 10.1104/pp.44.1.85.
3
The occurrence of nitrate reductase in apple leaves.硝酸还原酶在苹果叶中的出现。
Plant Physiol. 1969 Jan;44(1):110-4. doi: 10.1104/pp.44.1.110.
4
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Plant Physiol. 1965 Jul;40(4):691-8. doi: 10.1104/pp.40.4.691.
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Plant Physiol. 1960 Sep;35(5):700-8. doi: 10.1104/pp.35.5.700.
6
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Plant Physiol. 1954 Jan;29(1):98-9. doi: 10.1104/pp.29.1.98.
7
Independently segregating genetic loci concerned with nitrate reductase activity in Aspergillus nidulans.构巢曲霉中与硝酸还原酶活性相关的独立分离遗传位点。
Nature. 1963 Apr 20;198:262-3. doi: 10.1038/198262a0.
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Studies on nitrate reduction by Escherichia coli.大肠杆菌对硝酸盐还原作用的研究。
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Arch Biochem Biophys. 1954 Aug;51(2):379-94. doi: 10.1016/0003-9861(54)90493-2.
10
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