Weiblen R, Kreutz L C, Canabarro T F, Flores I E
Centro de Diagnóstico Veterinário, Universidade Federal de Santa Maria, Brasil.
Braz J Med Biol Res. 1991;24(8):773-5.
We describe an acute outbreak of balanoposthitis in bulls at an artificial insemination station in South Brazil. Bovine herpesvirus was isolated from preputial swabs in the Crandell feline kidney cell line and secondary fetal bovine lung cells and identified using the fluorescent antibody technique and electron microscopy. Polyclonal antibodies against the whole virus were used for identification of the bovine herpesvirus with the fluorescent antibody technique. Herpesvirus particles could be seen in the infected cells by electron microscopy. Eleven bulls had clinical signs resembling balanoposthitis and nine yielded virus.
我们描述了巴西南部一个人工授精站公牛发生的急性阴茎头包皮炎疫情。从包皮拭子中分离出牛疱疹病毒，该病毒在克兰德尔猫肾细胞系和二代胎牛肺细胞中培养，并使用荧光抗体技术和电子显微镜进行鉴定。使用针对全病毒的多克隆抗体，通过荧光抗体技术鉴定牛疱疹病毒。通过电子显微镜可在感染细胞中看到疱疹病毒颗粒。11头公牛出现类似阴茎头包皮炎的临床症状，9头检测出病毒。
