Horticulture Department, Purdue University, Lafayette, Indiana 47907.
Plant Physiol. 1970 Jan;45(1):19-24. doi: 10.1104/pp.45.1.19.
Evidences from experiments with stem sections of sunflower seedlings suggest that the transport of auxin may be limited by a restricted pool size of transportable auxin and restrictions in the availability of transport sites. A steady state of transport is observed over a range of lengths of stem sections, and over a wide range of auxin contents. The capacity of the sections to transport a pulse of auxin declines with aging after cutting, 50% decline occurring at about 10(+) hours; the transportability of a pulse of auxin declines rapidly after the completion of uptake, 50% decline occurring at about 1 hour. A chase treatment with unlabeled auxin does not alter transport, but a pretreatment with auxin depressed subsequent transport for about 1 hour. In depleted tissues such pretreatment is not inhibitory but rather is promotive of transport. The interpretation offered is that transport is limited by the pool size and transport sites, and roles for these factors are suggested in relation to the auxin transport gradient and the tropistic responses.
向日葵幼苗茎切段实验表明,生长素的运输可能受到可运输生长素的有限库大小和运输位点可用性的限制。在茎切段的一定长度范围内,以及在生长素含量的广泛范围内,都可以观察到运输的稳定状态。切段运输脉冲生长素的能力在切割后随时间的延长而下降,约 10(+)小时后下降 50%;脉冲生长素的运输能力在吸收完成后迅速下降,约 1 小时后下降 50%。用未标记的生长素进行追赶处理不会改变运输,但用生长素进行预处理会使随后的运输抑制约 1 小时。在耗尽的组织中,这种预处理不是抑制性的,而是促进运输的。提出的解释是,运输受到库大小和运输位点的限制,并且这些因素在生长素运输梯度和向性反应中起作用。