Department of Botany, Duke University, Durham, North Carolina 27706.
Plant Physiol. 1970 Jun;45(6):667-9. doi: 10.1104/pp.45.6.667.
The radial movement of oxygen in excised corn and jack bean roots was measured with a platinum wire electrode embedded in the root tissue. Measurements were made with the roots exposed to air and with the roots immersed in nutrient solution in the presence and absence of millimolar sodium azide. Effective rates of oxygen diffusion in the root tissue were also measured from 5 to 30 C and compared to the respiration rates of similar root segments over the same temperature range. Under conditions which allow the roots to exude freely, the interior of the root operates under an oxygen deficit. Inhibition of respiratory oxygen uptake by low temperature or azide treatment increased the flux of oxygen to the root interior.
用嵌入根组织的铂丝电极测量了离体玉米和豇豆根中氧气的径向运动。在根暴露于空气中和根浸泡在营养溶液中、存在和不存在毫摩尔叠氮化钠的情况下进行了测量。还在 5 至 30°C 下测量了根组织中氧气扩散的有效速率,并与同一温度范围内类似根段的呼吸速率进行了比较。在允许根自由分泌的条件下,根内部处于缺氧状态。低温或叠氮化钠处理抑制呼吸耗氧会增加氧气向根内部的通量。