Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92502.
Plant Physiol. 1970 Jun;45(6):719-22. doi: 10.1104/pp.45.6.719.
The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.
已在花椰菜（ Brassica oleracea ）花序匀浆的 20,000g 沉淀中发现负责合成胞苷二磷酸二甘油酯的酶的活性。用 0.02％Triton X-100 处理或冷冻和解冻后，沉淀中的酶活性更高。当反应混合物中包含氟化物时，酶的活性更高。该酶的 pH 最佳值为 5.6，Mn(2+)的最佳浓度为 1mm，Mg(2+)的最佳浓度为 30mm。在最佳浓度下，Mn(2+)比 Mg(2+)好 4 倍。通过添加肌醇可以增强生物合成的胞苷二磷酸二甘油酯的利用，从而形成磷脂酰肌醇。
