利用花椰菜酶制剂合成胞苷二磷酸二甘油酯。
Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower.
机构信息
Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92502.
出版信息
Plant Physiol. 1970 Jun;45(6):719-22. doi: 10.1104/pp.45.6.719.
The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.
已在花椰菜（ Brassica oleracea ）花序匀浆的 20,000g 沉淀中发现负责合成胞苷二磷酸二甘油酯的酶的活性。用 0.02％Triton X-100 处理或冷冻和解冻后，沉淀中的酶活性更高。当反应混合物中包含氟化物时，酶的活性更高。该酶的 pH 最佳值为 5.6，Mn(2+)的最佳浓度为 1mm，Mg(2+)的最佳浓度为 30mm。在最佳浓度下，Mn(2+)比 Mg(2+)好 4 倍。通过添加肌醇可以增强生物合成的胞苷二磷酸二甘油酯的利用，从而形成磷脂酰肌醇。
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