Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
Plant Physiol. 1970 Nov;46(5):641-4. doi: 10.1104/pp.46.5.641.
The flowering of Lemma perpusilla grown on half-strength Hutner's medium with sucrose under inductive photo-periods is inhibited in a periodic manner by daily transfers to water for short periods of time. The phase of maximal inhibition of flowering caused by water treatment is about 1 to 2 hours after the time of maximal sensitivity to light pulses. The rhythm of sensitivity to water treatments does not persist under continuous blue light. Supplementing the water with either Ca(NO(3))(2) or K(2)HPO(4) partially reverses the inhibition of flowering, with the first salt being more effective. Supplementation with NH(4)NO(3) or MgSO(4) increases the inhibition. The water effect on flowering is not observed in plants grown on half-strength Hutner's medium without sucrose. The water treatments may act by removing or destroying a crucial precursor for photoperiodic induction, with the other conditions modifying permeability. The system provides a new technique for investigating the mechanism of photoperiodic induction.
在诱导光周期下，生长在半强度 Hutner 培养基和蔗糖中的短葶山麦冬的开花以周期性的方式受到每日短时间转移到水中的抑制。由水疗引起的开花最大抑制的阶段大约是对光脉冲的最大敏感性之后 1 到 2 小时。在连续蓝光下，对水疗的敏感性节律不会持续。用 Ca(NO(3))(2)或 K(2)HPO(4)补充水可以部分逆转开花的抑制，第一种盐更有效。补充 NH(4)NO(3)或 MgSO(4)会增加抑制作用。在没有蔗糖的半强度 Hutner 培养基上生长的植物中观察不到水对开花的影响。水疗可能通过去除或破坏光周期诱导的关键前体而起作用，而其他条件则改变通透性。该系统为研究光周期诱导的机制提供了一种新技术。
