冻干菠菜叶绿体中光系统I活性对半乳糖脂的需求

Requirement of galactolipids for photosystem I activity in lyophilized spinach chloroplasts.

Krupa Z, Baszynski T

Biochim Biophys Acta. 1975 Oct 10;408(1):26-34. doi: 10.1016/0005-2728(75)90155-3.

Abstract

The effect of monogalactosyl diacylglycerol and digalactosyl diacylglycerol on reconstitution of Photosystem I activity in heptane-extracted and galactolipase-treated spinach chloroplasts was investigated. 2. Both galactolipids, in a molar ratio with chlorophyll of 2.5, partially restored Photosystem I activity in heptane-extracted chloroplasts. An addition of saturating amounts of plastocyanin caused complete reactivation of Photosystem I. 3. Similarly, with galactolipase-treated chloroplasts, both galactolipids partially restored Phostosystem I activity and additional amounts of plastocyanin were required for complete reactivation. 4. The action of galactolipids on partial reconstitution of Photosystem I supports the suggestion of their structural role in the restoration of thylakoid membranes.

摘要

研究了单半乳糖基二酰基甘油和双半乳糖基二酰基甘油对正己烷提取并经半乳糖脂酶处理的菠菜叶绿体中光系统I活性重建的影响。2. 两种半乳糖脂与叶绿素的摩尔比为2.5时，能部分恢复正己烷提取叶绿体中的光系统I活性。添加饱和量的质体蓝素可使光系统I完全重新激活。3. 同样，对于经半乳糖脂酶处理的叶绿体，两种半乳糖脂都能部分恢复光系统I活性，而完全重新激活则需要额外添加质体蓝素。4. 半乳糖脂对光系统I部分重建的作用支持了它们在类囊体膜恢复中起结构作用的观点。