Section of Genetics, Development and Physiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14850.
Plant Physiol. 1971 Mar;47(3):435-41. doi: 10.1104/pp.47.3.435.
Pea (Pisum sativum var. Alaska) and bean (Phaseolus vulgaris var. Red Kidney) stem sections treated with indoleacetic acid-1-(14)C, indoleacetic acid-2-(14)C, and indoleacetic acid-5-(3)H were homogenized, extracted with phenol, and the water-soluble, ethanol-insoluble material subjected to further fractionation. Following an 18-hour incubation period in indoleacetic acid-1-(14)C, most of the label was found as nonindole-(14)C in high molecular weight polysaccharide, as phenol extraction is specific for both RNA and polysaccharides. With indoleacetic acid-2-(14)C and -5-(3)H, and to a lesser extent with indoleacetic acid-1-(14)C, radioactive indoles were obtained by hydrolysis from a heterogeneous fraction between about 500 and 30,000 molecular weight, possibly polysaccharide in nature. Indoleacetic acid accounted for 8% and indole aldehyde accounted for 21% of the total radioactivity in the extract.
豌豆（Pisum sativum var. Alaska）和菜豆（Phaseolus vulgaris var. Red Kidney）茎段用吲哚乙酸-1-(14)C、吲哚乙酸-2-(14)C 和吲哚乙酸-5-(3)H 处理后匀浆，用苯酚提取，水溶性、乙醇不溶性物质进行进一步分级分离。在吲哚乙酸-1-(14)C 孵育 18 小时后，大部分标记物作为非吲哚-(14)C 存在于高分子量多糖中，因为苯酚提取对 RNA 和多糖都具有特异性。用吲哚乙酸-2-(14)C 和 -5-(3)H，在较小程度上用吲哚乙酸-1-(14)C，从约 500 到 30000 分子量的异质部分中通过水解获得放射性吲哚，可能具有多糖的性质。吲哚乙酸占提取物中总放射性的 8%，吲哚乙醛占 21%。
